Luciferase plasmid yields

W.H. Colledge whc at
Tue Jul 16 16:00:40 EST 1996

We are having difficulties obtaining good yields of a CMVluciferase
plasmid grown in DH5 alpha. Typically we only obtain about 50ug from 300ml
TB cultures. Has anyone had similar problems? Is breakthrough luciferse
expression toxic to E.Coli? Many thanks.

Bill Colledge
whc at

Physiological Laboratory
University of Cambridge
Cambridge CB2 3EG

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