Help: PCR problems with D12S99 Just wondering if anyone else has been having problems with their primer set for the D12S99 locus (12p13 region). We have been working with the probe AFM217xa7a/AFM217xa7m and have had no success, after having tried all of the following conditions: Annealing temp: 50, 55, 60 Initial Melting temp: 92, 94 Mg2+ conc: 1.5 - 4.0 mM Primer conc: 1X, 3X Glycerol/no glycerol Any more suggestions??? Thanks!!
wolffie at u.washington.edu
Tue Jul 16 14:57:24 EST 1996
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