Help: make primers from amino acid sequence

Paul N Hengen pnh at kaylor.ncifcrf.gov
Tue Jul 16 11:23:20 EST 1996


Phil Robinson (mdpjr at cc.newcastle.edu.au) wrote:

> Could anyone please suggest  a program to help design primers from
> fragments of amino acid sequence.  We find its relatively easy to find
> programs to help work from DNA, but not so simple from protein
> sequence.  The specific problem is that we have purified a protein,
> have then obtained 5 or 6 segments with about 8-12 amino acids in
> each, and now want to start PCR as a step to cloning.  Many primer
> design packages seem not to be of help, but maybe we have not found
> the right place to look.

Try PrimerGen written by John Nash (1993). A computer program to calculate and
design oligonucleotide primers from amino acid sequences. CABIOS 9: 469-471.
You can go through the link on my homepage or download it directly from:

http://cansnd.cisti.nrc.ca/~nash/john.nash.html

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