Lac operon curing?
rand at pelican.dbe.csiro.au
Wed Jul 17 04:34:22 EST 1996
In article <Pine.SOL.3.91.960716133542.27124A-100000 at design.eng.uci.edu>,
Doug Pecota <pecota at design.eng.uci.edu> wrote:
> I was told that there is a method for selecting cells which lack the
> lac operon by killing cells that contain it?
TONPG (orthonitrophenyl beta D thiogalactoside) can be used to select lacY
minus mutants - included will be deletions of the operon.
If the cells are gal E minus then one can select for resistance to lactose.
Keith Rand, Sydney Australia
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