Lac operon curing?

Keith Rand rand at pelican.dbe.csiro.au
Wed Jul 17 04:34:22 EST 1996


In article <Pine.SOL.3.91.960716133542.27124A-100000 at design.eng.uci.edu>,
Doug Pecota <pecota at design.eng.uci.edu> wrote:

>      I was told that there is a method for selecting cells which lack the 
> lac operon by killing cells that contain it? 

TONPG (orthonitrophenyl beta D thiogalactoside) can be used to select lacY
minus mutants - included will be deletions of the operon.
Alternatively,
If the cells are gal E minus then one can select for resistance to lactose.

-- 
Keith Rand,  Sydney Australia



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