kertesz at micro.biol.ethz.ch
Thu Jul 18 13:20:24 EST 1996
Touchdown gives me much better specificity, especially when the two
primers have Tm's that are way apart. I start at an annealing temp. about
10-15 deg. too high, then come down with it one degree per cycle, before
finally running a "normal" PCR for 15-25 cycles at the end. We have a
Trio-block, though, where it is dead easy to program, though...
Philippe Lefebvre wrote:
> Hi everybody!
> Does anyone has already used touchdown pcr, and could say more about pros and cons
> about this method. May be is there even a protocol available out there?
> Thanks in advance for your help!
> Dr Phil Good
CH-8092 Zurich, Switzerland
tel: +41-1-632 3357
fax: +41-1-632 1148
e-mail: kertesz at micro.biol.ethz.ch
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