Depc water

Thomas Thomas at hastingslab.harvard.edu
Thu Jul 18 10:06:51 EST 1996


In article
<Pine.A32.3.92a.960717153412.31721A-100000 at homer18.u.washington.edu>,
chusj at U.WASHINGTON.EDU (Shi-Jye Chu) wrote:

> Hi:
> 
>     Does anybody know how to make up Depc water ?  thanks a lot
> 
> 
> 
> 
> 
>    S-J

We routinely add 0.1-0.2% (v/v) DEPC to water, dissolve it and incubate it
overnight, then autoklave (>30 min) to inactivate remaining DEPC.

Thomas

-- 

Sender:  Thomas Urbig
         Harvard University
         Institute for Molecular and Cellular Biology
         16 Divinity Ave.
         Cambridge, MA 02138
         Tel: (USA) 617 495 3716;   Fax:  (USA)  617 496 8726
         Email:  Thomas at hastingslab.harvard.edu



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