Depc water

Douglas Prasher dprasher at capecod.net
Thu Jul 18 11:42:24 EST 1996


Shi-Jye Chu wrote:
> 
> Hi:
> 
>     Does anybody know how to make up Depc water ?  thanks a lot
> 
>    S-JYears ago, when I was working with RNA, I made DEPC-treated water in the 
following manner.

Add DEPC (dangerous stuff, so work with it in a good hood) to 0.2% to 
your water (in glass, not plastic).  Stir or shake 1-2 hours, then 
autoclave to remove residue DEPC.

Douglas Prasher



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