Depc water

Douglas Prasher dprasher at
Thu Jul 18 11:42:24 EST 1996

Shi-Jye Chu wrote:
> Hi:
>     Does anybody know how to make up Depc water ?  thanks a lot
>    S-JYears ago, when I was working with RNA, I made DEPC-treated water in the 
following manner.

Add DEPC (dangerous stuff, so work with it in a good hood) to 0.2% to 
your water (in glass, not plastic).  Stir or shake 1-2 hours, then 
autoclave to remove residue DEPC.

Douglas Prasher

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