EMSA-protocol: Additions and error correction

Heinz-Juergen Schaefers mfm434 at rzmail.uni-erlangen.de
Thu Jul 18 13:36:28 EST 1996


Hi all!

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1. Error correction:

-   measure protein content:
		5 µL supernatant
		75 µL H2Odd
		20 µL BioRad dye
                (as standard: 0-10 µg/mL BSA in H2Odd)
                              ^^^^^^^^^^^^^^^^^^^^^^^
                              should be: 0-10 µg BSA in 80 µL H2Odd

2. Addition:

all solutions/buffers exept solution A and C should be stored at -20°C
*****************************************************************************

sorry!

bye
	Heinz
-- 
Dipl. Biol. Heinz-Juergen Schaefers
Universitaet Erlangen-Nuernberg / Med. Klinik IV / Nephrologisches Forschungslabor
Loschgestr. 8, D-91054 Erlangen, Germany
E-mail: dienstl.: mfm434 at rzmail.uni-erlangen.de / priv.: h-j.schaefers at erlangen.netsurf.de



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