260/280 is 1.6 Good enough?

Richard Van Frank vanfrank at iquest.net
Fri Jul 19 10:16:01 EST 1996


In article <31ED2EC7.55AC at unity.ncsu.edu>,
   Susan Hogarth <sjhogart at unity.ncsu.edu> wrote:
>Hi,
>Sambrook et al. says "pure preperations of DNA...have OD260/OD280 
>values of 1.8..." I spec'ed a sample and got a ratio of 1.6. Is this 
>OK? 
>
>Thanks,
>Susan
Depends, I would look at the pattern of the scan to see how sharp the 260 peak 
is and to see if there is a peak at 280, or any other wavelength. It is risky to 
rely on a ratio without looking at the scan.
RMVF



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