Separate ss/ds DNA

Fri Jul 19 16:59:37 EST 1996

On Thu, 18 Jul 1996, Susan Jane Hogarth wrote:

> Hi, this may be too broad a question, but I'll ask anyway.
> I want to seperate small amounts of dsDNA from ssDNA for Cot analysis
> (so I only need to keep the dsDNA). I know (think!) I can use TCA precip
> after digestion of the ssDNA, or HAP columns, but I wondered if there
> are other methods and which one people prefer. Thanks for any info.
> -- 
> Susan Jane Hogarth
> Man: What is my purpose?
> Universe: Define purpose.

How about CsCl gradient?

			  Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at

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