Touchdown pcr

Olivier Gandrillon ogandril at
Fri Jul 19 06:49:55 EST 1996


I even found a better protocol that's derived from touchdown but much 
more simple to program and more robust (one program amplifies it all!): 
stepdown PCR (Hecker, 1996; BioTechniques 20:478-485). I am now usiong 
it on a regular basis and am perfectly satisfied with it!


Philippe Lefebvre wrote:
> Hi everybody!
> Does anyone has already used touchdown pcr, and could say more about pros and cons
> about this method.  May be is there even a protocol available out there?
> Thanks in advance for your help!
> Dr Phil Good

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