PCR using a primer with a dUTP

Dr. Duncan Clark duncan at genesys.demon.co.uk
Sat Jul 20 05:05:47 EST 1996

In article <4si858$s2p at ukwsv3.ggr.co.uk>, Fraser Dr N J
<njf16154 at ggr.co.uk> writes
>I don't know if this is related, but I posted a similar problem a short time
>ago (and have had no answers). This time I was using primers containing dITP.
>However, these worked fine with Taq , but got nothing with Pfu. What polymerase
>are you using ? You may get some success if you try another one.

The proof-reading pols such as Pfu, Pfu, Vent and Deep Vent do not like
to incorporate dUTP.


My mind's made up. Don't confuse me with the facts!
Duncan Clark
GeneSys Ltd.

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