I need a good cosmid isolation protocol
leach at bu.edu
Mon Jul 22 14:01:42 EST 1996
i had problems with the qiagen protocol several years ago.
In the end I used the old-fashioned miniprep protocol...and subjected the mix
to several phenol/chloroform extractions to clean it up. Finally, ppt'd the
DNA with etoh......any old miniprep protocol using phen/chlo should work..
Michael R. King (king1 at students.uiuc.edu) wrote:
: Can anyone give me a reference to a protocol giving high yield cosmid
: isolation. I've used the kits like Qiagen but they don't work very well.
: I'm doing a basic mini-prep as I type this but I thought I would get a
: little advice.
: Kind regards,
: Michael R. King
..... Martin Leach Email:leach at bu.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
_/ |-/__==/ 80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118 "Not the old underpants
USA on your
My homepage: http://220.127.116.11/Leach.html
Biotech company WWW REgistry has moved to: http://www.data-transport.com
Check out the Biotech Rumor mill: http://18.104.22.168/jumper/rumor.html
More information about the Methods