Blotting of PFGE

WSchick at aol.com WSchick at aol.com
Mon Jul 22 22:19:26 EST 1996


Try anal biochem ishihara 184, 207-212 1990 electroblotting of ds dna
transfer in 10 minutes.
He uses a 1/20 th concentration of bufffer, soaks the gel a little to reduce
its conductivity then uses high power to push out the big DNA    You could
probably do this in a conventional Transphor buffer tank from Hoefer as long
as your recirculated the buffer..  You should not use a semi dry blotter as
there wouldnot be enough buffer compoents.

Let me know about your success.

Walt Schick



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