[Q] Sonication times for E. coli?

Ned Mantei mantei at neuro.biol.ethz.ch
Wed Jul 24 00:15:57 EST 1996


In article <4t4316$793 at decaxp.HARVARD.EDU>, timur at beagle.colorado.edu wrote:

> Hello.  How long should one sonicate E. coli cells for in order to
> break down most cells

1) Sonicate until the suspension has a "dirty dishwater" look, instead of
looking sort of "creamy".
2) Take 100 ul aliquots at various times, including before you start.
Centrifuge 1 min. in Eppendorf centrifuge. As cells break the pellet gets
smaller.

-- 
Ned Mantei
Dept. of Neurobiology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
mantei at neuro.biol.ethz.ch   Fax: +41-1-633-1046



More information about the Methods mailing list