igrothd at INFO.CURTIN.EDU.AU
Tue Jul 23 05:29:11 EST 1996
Can anyone suggest a cure for stutter bands when analysing
dinucleotide microsatellites by PCR. We are developing several
microsatellites for use in population studies in a fresh water
crustacean. The loci identified so far all have very large repeat
regions eg CA(50-70 repeats). These are usually perfect although they
can be complex.
When we do PCR we get 5-8 bands displaced 2bp in a ladder formation.
This is a greater problem than I have seen in other species eg human.
We have tried using DMSO, increasing the annealing temp, Taq gold
without removing the problem. I have thought of using deaza-GTP
instead of dGTP. Has anyone tried this?
Dr David Groth
School of Biomedical Sciences
GPO Box U1987
Perth Western Australia
igrothd at info.curtin.edu.au
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