help for HPLC anionic

mazella at naxos.unice.fr mazella at naxos.unice.fr
Wed Jul 24 10:46:44 EST 1996


I am trying to separate nucleotides with a columm called NUCLEOSIL 100-5 SA
from machery-nagel ( it's a strong anion-exchange columm ).
The solvent is KH2PO4 50 mM-1000 mM pH 5,6 in 45 mns.

The problem is that everything is eluted in the 5 first minutes without any
separation.
I try to separate GMP, GDP, GTP, GP4G, and ADP.

I would appreciate some help.
Thanks for your time.
Claude

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