[Q] Sonication times for E. coli?

Richard Van Frank vanfrank at iquest.net
Wed Jul 24 21:34:06 EST 1996


In article <4t4316$793 at decaxp.HARVARD.EDU>,
   Timur Shtatland <berriz at husc.harvard.edu> wrote:
>
>
>
>Hello.  How long should one sonicate E. coli cells for in order to
>break down most cells, and fractionate contents by molecular weight?
>Contrary to what I would have expected, I found that the amount of
>protein released does not plateau within the first 20 min. of
>sonication.  What are typical sonication times in your experience?
>
>Thank you very much in advance,
>
>Tim
The time required is dependent on the power output of the sonicator, the 
suspending medimum and the cell concentration. Monitor the process using a 
microscope, you should see fewer and fewer whole cells.
RMVF




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