Use of triethylamine to elute phage

Alex Chang lpss at unixg.ubc.ca
Thu Jul 25 14:44:49 EST 1996


I have a protocol from a paper for immuno-panning using Nunc's
immunotube. For the phage elution, triethylamine is used to incubate for
10 min, followed by the addition of certain amount of 1 M Tris-HCl, pH
7.4. I am wondering anyone using triethylamine for this purpose, and what
is the mechanism of its use.

Thanks

Alex Chang
Pathology
University of British Columbia
achang at hivnet.ubc.ca





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