Green Bacteria Cultures

Darren Boehning boehnin1 at
Thu Jul 25 08:30:47 EST 1996

Has anyone had the experience of overnight cultures in LB/AMP (DH5alpha)
turning green?  Sometimes we see it, other times not.  If I make up
several o.n. cultures in 1X LB, usually only one will turn green
(literally bright, algae-looking green).  Anyone seen this before?  It is
actually somewhat interesting.  By the way, we dilute from 5X LB stock
every time we make up a culture.
Thanks in advance for thoughtful insights,
Darren Boehning
Thomas Jefferson University
Department of Pathology
boehnin1 at

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