help for HPLC anionic

Christoph Grunau cgrunau at imb-jena.de
Thu Jul 25 09:07:59 EST 1996


In article <4t5gh4$541 at mserv1.dl.ac.uk>, <mazella at naxos.unice.fr> wrote:

> I am trying to separate nucleotides with a columm called NUCLEOSIL 100-5 SA
> from machery-nagel ( it's a strong anion-exchange columm ).
> The solvent is KH2PO4 50 mM-1000 mM pH 5,6 in 45 mns.
> 
> The problem is that everything is eluted in the 5 first minutes without any
> separation.
> I try to separate GMP, GDP, GTP, GP4G, and ADP.
> 
Try a gradient of
buffer A: 20mM TRIS pH 8.2
buffer B: 20mM TRIS pH 8.2, 1 M NaCl
from 0% B to 10% B in >10 min

It works well with the strong anion-exchange column from Pharmacia I use.

Good luck - Christoph

-------------------------------------------------------------
Christoph Grunau
AG Genomanalyse
Institut fuer Molekulare Biotechnologie e.V.
Beutenbergstr.11
D-07745 Jena
Germany

Tel.: +49 (03641) 65 62 42
Fax.: +49 (03641) 65 62 55



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