silver staining...

Sailesh Surapureddi Sailesh.Surapureddi at mcb.liu.se
Sun Jul 28 15:27:11 EST 1996


Hi
I suppose you could de-stain it with Pot. dichormate, and then repeat the
whole process again.  if that doesnt work, here is a used but not
appreciated method. Transfer your gel to a NC and  expose your blot to some
(any available Ab in the lab) Ab and then cross react with Sec. Ab linked
to HRP. Develop with ECL, one draw back with ECL is, it cross reacts with
all bands on the blot and this could be exploited  to your advantage.

Sailesh.

Dr.Sailesh Surapureddi
Plan 12; Inst. for Cellbiologi
Halsouniversitetet
S 581 85, Linkoping
Sweden.

Tel: +46-13-223917 (W) +46-13-138839 (R)
Fax: +46-13-224149
E.mail: Saisumcb at hulio.liu.se
        Saisu at mcb.liu.se





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