about DNA recovery from agarose gel

Eitan Rubin bcrubin at dapsas1.weizmann.ac.il
Sun Jul 28 08:01:13 EST 1996


In article
<Pine.ULT.3.91.960716114331.9143C-100000 at raphael.acpub.duke.edu>, Ke Wei
<victorke at acpub.duke.edu> wrote:

> On 10 Jul 1996, Jin-seon Im wrote:
...
> You don't just precipite the PCR products directly because of purity 
> concerns, especially when you are cloning genes.

If you just need to sequence the product, and you don't need a world
record in sequence quality (for example when you want to verify you got
the right product, or you want to localize a deletion), you *do* just EtOH
precipitate PCR products. Works every time for me.

-- 
Eitan Rubin, Plant Genetics, Weizmann Inst. of Science
Phone: (972)-8-9342421
Email: bcrubin at dapsas1.weizmann.ac.il



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