MMTV PROMOTER

[Ferland Louis H.]

On Tue, 9 Jul 1996 annalise at medmicro.uct.ac.za wrote:

> Date: Tue, 9 Jul 1996 11:51:08 UNDEFINED
> From:annalise at medmicro.uct.ac.za
> To: methods at net.bio.net
> Subject: MMTV PROMOTER
> 
> We are having problems inducing the expression of genes under the control of 
> an MMTV promoter.  Can anyone give us some ideas on how to get the system 
> working?
> 
> Thanks
> 
> Anna-Lise Williamson
> 

Anna-Lise,

Presumably, you are looking at induction by glucocorticoids. What cell 
type and specific line are you using? Are you certain they have 
glucocorticoid receptors? We just had that problem a few months ago and 
solved it by co-transfecting a GR expression vector.

Just a thought.


Dr. Louis H. Ferland
Centre de Recherche, Hotel-Dieu de Montreal
Dept de Nutrition, Universite de Montreal
Phone: (514) 843-2757     FAX: (514) 843-2719