"Best" Long PCR

varyc.mmcwp4 at mmc.org varyc.mmcwp4 at mmc.org
Mon Jul 29 08:15:41 EST 1996

Hi all, 
Does anybody have any strong opinions on the best long
PCRprotocol(s):we need to amplify a  23kb stretch of human genomic
DNA. Any particulars regarding primer design tricks and/or  tried and true
kits as well as homebrews would be much appreciated.

Thanks in advance for any help.

Cal Vary

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