Transfecting A549 cells

Dima Klenchin klenchin at facstaff.wisc.edu
Tue Jul 30 00:01:55 EST 1996


In article <9607291922.AA35057 at echo.i-link.net>, RAWnHDG at aol.com ("Robin Ann Wasserman, Ph.D.") wrote:
#Hi,
#
#I'm having a lot of difficulty transfecting A549 cells...
#
#I've tried:  Calcium phosphate, electroporation and a variety
#of commercially available lipids.
#
#Does anybody have any hints/tips/tricks to get them to
#transfect?
#
#Note:  I can successfully transfect 293 cells by all these
#methods as a control for my 'technique'.

What is A549? Are they by any chance related to A431?
I've transfected latter with retroviral vectors and it didn't seem to be
any difficult...

- Dima





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