DNA purification

Michael Kloth mtkloth at facstaff.wisc.edu
Tue Jul 30 19:47:17 EST 1996


Hi.

After my last couple of overnight proteinase K digestions, my yield of DNA 
after precipitation is quite low.  (Prior to proteinase K digestion I have 
nice visible DNA pellets.)  I have not changed any part of my protocol and I 
am still using the same lot of Boehringer Mannheim proteinase K.  Does anybody 
have any suggestions as to what my problem might be?  Thanks in advance




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