ScFv expression in pVIII-coat protein??????

Danny T M Leung b574713 at mailserv.cuhk.hk
Wed Jul 31 09:01:45 EST 1996


Dear phage-library workers'
        I would like to know whether ScFv-antibody normally expressed in pIII protein can 
be expressed in pVIII protein. If not, are there any ways to increase ScFv-antibody copy
number per phage which is about 1-5 copies per phage in the case of pIII protein? The
problem I found is the ScFv-Ab phage derived from my hybridoma  binds only weakly to
the antigen.



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