SDS PAGE band blurring

Wed Jul 31 20:34:24 EST 1996

I am not sure if it could explain the band blurring, but I do know that a 
sample that is too acidic will cause the bpb loading dye to turn yellow. 
A few drops of a pH8 Tris buffer will restore the blue color.  From this, 
maybe there is a pH change across your gel for some reason???
good luck

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