Methods July 1996 Archives by author

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Starting: Mon Jul 1 02:21:43 EST 1996
Ending: Wed Jul 31 23:42:19 EST 1996
Messages: 1067

• Biotin dUTPs   "DAVID O'CONNELL STAFF", AGRIC. & VET. DEPT.
• site directed mutagenesis kits   "Miss. V.M. Hodges", by way of florigen at biotech.ufl.org Florida Genetics Corporation
• Use of RIA human insulin with ovine plasma   Value is fixed: using "Sandra.Christiansen"
• Interupted PCR   Shane Atwell %Kuntz
• Staining gels in a microwave   Shane Atwell %Kuntz
• ECL direct with oligos   Juergen Sasse (Path)
• Expression of toxic proteins:  
• Expression of toxic proteins:  
• Bibliography Software Recommendations  
• FORMALIN AND DNA extraction  
• Ethidium question  
• unbiotinylating streptavidin ... ?   Keith Hoek -BIOCHEM
• xylose reductase   84075
• xylose reductase   84075
• In situ hybridization   K J C 944
• Using ClaI for subcloning   :-Peter
• dextrose syrup--resins   Mar ía de Fátima González
• Source for KlenTaq?   A.J.Cann
• Protocols for determining autophosphorylation in crude plant protein extracts   AFRS
• inducible mammalian promoter   NETSCAPE APPLICATION
• SDS PAGE band blurring   NETSCAPE APPLICATION
• Non-radioactive Tyr-kinase assays?   AShaw5
• [Q] Sonication times for E. coli?   AShaw5
• BIAcore ligand fix   AShaw5
• BIAcore ligand fix   Yasuhito Abe
• IMPORTANT - net.bio.net down this weekend!!   BIOSCI Administrator
• New BIOSCI Sun server is online!   BIOSCI Administrator
• IMPORTANT - METHODS newsgroup index is fixed!   BIOSCI Administrator
• IMPORTANT - BIOSCI maintenance downtime 21 July   BIOSCI Administrator
• IMPORTANT - BIOSCI Fundraising Update!   BIOSCI Administrator
• BIOSCI Archives Backlog   BIOSCI Administrator
• poly A+ purification   Adriana
• Frequency of BstE II cutting?   Mikhail Alexeyev
• chloramphenicol concentration   Mikhail Alexeyev
• Rifampicin activity   Mikhail Alexeyev
• P1 transductions   Mikhail Alexeyev
• unbiotinylating streptavidin ... ?   Mikhail Alexeyev
• Luciferase plasmid yields   Mikhail Alexeyev
• 260/280 is 1.6 Good enough?   Mikhail Alexeyev
• Using ClaI for subcloning   Mikhail Alexeyev
• Transposon Mutagenesis HELP PLEASE   Mikhail Alexeyev
• RE digest X - Ligation - RE Digest Y (purif. needed?)   Javier Alzerreca
• Epitope Tagging Antibodies   Thomas R. Anderson
• Nuclear extracts from peripheral blood lymphocytes   Kristin B. Andersson
• Fos/Jun antibodies   Jennifer Anklam
• Epilepsy: neuronal ultrastructure   Dr. T. Anoopkumar
• ULTRA STRUCTURE OF HUMAN BRAIN   Dr. T. Anoopkumar
• NEB Alkaline phosphatase contain Nuclease?   Tracy Aquilla
• Cutting glass for sequencing plates?   Arioch
• running extracts down hi-trap columns...   Arioch
• silver staining...   Arioch
• Ago: AA analysis problem   Jorge Gonzalez Arroyo
• AA analysis   Jorge Gonzalez Arroyo
• protein-protein interactions in vivo   Technical Assistance
• endotoxin kit   Technical Assistance
• Primer extension protocol   Vinod Asundi
• Tetracycline inducible system   Graham Atherton
• Northern with DIG   Chanok Avirutdhakarn
• protein-protein interactions in vivo   Jo=?iso-8859-1?Q?=EBl BAGUET
• protein-protein interactions in vivo   Jo=?iso-8859-1?Q?=EBl BAGUET
• Query about phage   SPIC BIOPROCESS
• Subtractive cDNA   Veronique BOYER
• Repair of MJ Resrch PTC-100 (PCR machine)   Dan Baggott
• 4 way ligation avoided !!!!!!   Camella Bailey
• 4 way ligation avoided !!!!!!   Camella Bailey
• Staining gels in a microwave   David Bailey
• Gibco/BRL's web adress   Roger Baird
• site directed mutagenesis kits   little sausage called Baldrick
• ? about DNA extraction kits   Carol Barrett
• Wizard preps for "advanced" uses   Peter Barrett
• Wizard preps for "advanced" uses   Peter Barrett
• Streck fixative for in situ   Angeline Bartholomeusz
• Antibodies directed against interferon   Angeline Bartholomeusz
• Linscott alternative?   L. Bastian
• rat brain library   Robert Bateman
• Problems with fmol   Dr. C. Baumstark-Khan
• tubes for in-situ hybridization   Anne Bazanowski
• Staining gels in a microwave   Ed Beaty
• PRIMERS   Andreas Becker
• Mac scanner for blots...   Robin Beech
• PCR Blood group test   Robin Beech
• Concentration of DNA in Ligations???   M C Behrendt
• 5' nucleotide addn.   Brett Beitzel
• 5' nucleotide addn.   Brett Beitzel
• Poly C PCR, anyone?   Brett Beitzel
• About Euro Taq Patent   Brett Beitzel
• exons & protein domains   R M Bernstein
• geneclean: vortexing   Trevor Bezdek
• dna prep for transfection of 293 cells   Trevor Bezdek
• photodocumentation system?!   Biomol
• Are there labs for rent?   Allen Black
• Freezing Baculo virus; what happens?   Allen Black
• Wanted: buffer for pH 2.5-3!!   Shaun D. Black
• Ni-NTA/HiTrap-Chelating, urea, the pH, and thiol reagents   Wulf Blankenfeldt
• quantitative PCR   Volker Blaschke
• Green Bacteria Cultures   Darren Boehning
• Karen Browning?   Peta C. Bonham-Smith
• SmaI digest   TL. Borgeaud
• Mint genome size   Alex Borovkov
• Using ClaI for subcloning   Joe Boutell
• four-way ligation help !!!   Robot Boy
• 4 way ligation avoided !!!!!!   Robot Boy
• salt conc. and DNA mobility question   Robot Boy
• Linscott alternative?   J. Boyce
• Custom oligosynthesis and custom protein sequencing   Joan Boyce
• Biotin dUTPs   Joan Boyce
• Frequency of BstE II cutting?   Chris Boyd
• RNASE DECONTAMINATION REVIEW   Chris Boyd
• blue colonies-no Xgal   Chris Boyd
• Alpha-complementation in mammalian cells?   Chris Boyd
• Frequency of BstE II cutting?   Chris Boyd
• Something living on colchicine!   Chris Boyd
• Anti HA ab source?   Chris Boyd
• ERRATUM: PFGE to separate 20-50 kb   Chris Boyd
• Is KlenTaq commercially available?   Scott K. Boyer
• How long does DNA last?   Ian Brandt
• four-way ligation help !!!   John Brennand
• 4 way ligation avoided !!!!!!   John Brennand
• Wanted: supF bacteria   John Brennand
• removal of imidazole   John Brennand
• European Radiochemical suppliers   John Brennand
• exons & protein domains   Steven Brenner
• NAOMI Software   Simon M. Brocklehurst
• QIAexpress Detection Systems   Lesley Brown
• Something living on colchicine!   Alan Brownlee
• Adding 3' terminal thymidine to blunt ended DNA   Sebastian Bunka
• Electroporation - need a biotechniques copy   Sebastian Bunka
• Nuclear population marker?   Bill Burnett
• Ficoll removal from organelle lysates   Dr E. Buxbaum
• Detergents   Dr E. Buxbaum
• ELISA? Description please....   Dr E. Buxbaum
• Native PAGE of very basic small protein   Dr E. Buxbaum
• geneclean: vortexing   CAShug
• cDNA library plating   CSJGH at leeds.ac.uk
• LAB-ANIMAL RES.EQUIP.CATALOG ON WEB   JAN CZEKAJEWSKI
• in situ PCR course   Diego Cantalapiedra
• Uniequip DNA Workstation - comments?   Jeremy Carson
• MJ-Research DNA Engine   Michael Casey
• An assay for alpha ketoglutarate dehydrogenase activity   Ron Caspi
• Blue color comes late lately in yeast 2 hybrid   Edward K.L. Chan
• Use of triethylamine to elute phage   Alex Chang
• SDS PAGE band blurring   Frances E. Chen
• Sequencing gels   Nikolai Chitaev
• subcloning pcr bands   Joe Chou
• Depc water   Shi-Jye Chu
• Wanted: buffer for pH 2.5-3!!   Namjin Chung
• Which determines mobility?   Namjin Chung
• Enterovirus RT-PCR primers   Nanto Cielens
• RNA extraction from paraffin bloks   Corrado Cilio
• It is possible to get RNA from paraffin blocks?   Corrado Cilio
• ThermoSequenase   Dr. Duncan Clark
• E.coli and toxic proteins   Dr. Duncan Clark
• SmaI digest   Dr. Duncan Clark
• Help regarding availability of Mwo I restriction enzyme   Dr. Duncan Clark
• 4 way ligation avoided !!!!!!   Dr. Duncan Clark
• ThermoSequenase   Dr. Duncan Clark
• temperature induction of BACTERIA   Dr. Duncan Clark
• Expression of toxic proteins:   Dr. Duncan Clark
• P1 transductions   Dr. Duncan Clark
• PCR of bugs in berries   Dr. Duncan Clark
• Expression of toxic proteins:   Dr. Duncan Clark
• Poly C PCR, anyone?   Dr. Duncan Clark
• Taq polymerase patent (not)   Dr. Duncan Clark
• Colony picking/gridding machine   Dr. Duncan Clark
• ALF/LICOR Sequencers   Dr. Duncan Clark
• PCR using a primer with a dUTP   Dr. Duncan Clark
• About Euro Taq Patent   Dr. Duncan Clark
• Touchdown pcr   Dr. Duncan Clark
• Electroporation - need a biotechniques copy   Dr. Duncan Clark
• Bubble-getters   Dr. Duncan Clark
• Sonication Protocol!!   Dr. Duncan Clark
• [Q] Sonication times for E. coli?   Dr. Duncan Clark
• Stutter Bands   Dr. Duncan Clark
• P1 transductions   David Clarke
• gradient pcr?   Brent Cochran
• Luciferase plasmid yields   W.H. Colledge
• Rapid hybridization buffer   Martin Collinson
• Transcription factor binding sites   Martin Collinson
• Hybond N   Martin Collinson
• PCR Machines - Suggestions?   Seth Corey
• Lambda Pop6   CoreyKeith
• Yeast genome data bank access   Jose Miguel Lopez Coronado
• ELISA with 4-chloro-1-naphthol   Fred Correia
• anti-6Xhis antibody - any new info?   Maria C. Costanzo
• LONG PCR   Michael B. Coulthart
• long range PCR of genomic insert in Lambda   Michael B. Coulthart
• ELISA? Description please....   Rob Cowie
• Green Bacteria Cultures   Albert Crescenzo
• TA Cloning with Elongase   Joe Crouse
• EGF ELISA   Mickey Croyle
• exons & protein domains   David Cullinan
• RNA for Differential Display? HELP!!   Mike Curtin
• LDL receptor plasmids   Marc DIEU
• Biotin dUTP's   DOCON at ollamh.ucd.ie
• QIAGEN MINIPREP ROBOT   DSF
• Help for electroporation of E.coli starved cells   Bregeon Damien
• long PCR?   Bregeon Damien
• Need protocol on caged cAMP   Dr. L. Darjania
• Thermocyclers   Gottfried Dasen
• gradient pcr?   Gottfried Dasen
• Is KlenTaq commercially available?   David L. Haviland, Ph.D.
• Using ClaI for subcloning   David L. Haviland, Ph.D.
• Mol Bio Peer Review Group   David W. Bradley, Ph.D.
• Rep : Elisa for Q-RT-PCR   Mr SHIRE David
• Qiagen plasmid maxi kit; low yields   Michael Daws
• How to sequence primers?   Tony Day
• JOB listings!!!! Web page BIOMED JOBS IN CANDA   Graham Dellaire
• Statistical Calculation (Help! is my reasoning okay!!)   Graham Dellaire
• Biomedical Job Listings from ExpMed (McGill University)   Graham Dellaire
• RAPD   Jacqueline Delp
• Hybond N   Rex Denton
• Ligation with two base fill-in   Rosa Deves
• Sequencing gels   Roger Dewar
• Formalin and DNA extraction   Roger Dewar
• Thank you...re Sequencing gels   Roger Dewar
• amplification problem   Gabriel E. DiMattia
• Commercial sequencing costs????   Gabriel E. DiMattia
• GpppG cap and oocytes?   Paul Digard
• silver staining...   Paul Digard
• dicistronic vector   John Dixon
• Wanted: supF bacteria   John Dixon
• IRES-Element   John Dixon
• long range PCR of genomic insert in Lambda   John Dixon
• DNA ligation   Dr Andrew J. Doherty
• Average molecular weight of a DNA base pair   Dr Andrew J. Doherty
• quantitative PCR   Dr Andrew J. Doherty
• 260/280 is 1.6 Good enough?   Dr Andrew J. Doherty
• Oxalyticase in the UK?   Fergus Doherty
• PCR Machines - Suggestions?   Alec Dolan
• polyisopren   Anke Doller
• Methods & Reagents Newsgroups   Guivarc_h Dominic
• Sonication Protocol!!   Tom Dougherty
• Bubble-getters   Christopher Dubay
• Post-ligation restriction   Richard J. Dudley
• DNA ligation   Richard J. Dudley
• Bibliography Software Recommendations   Craig Echt
• Transposons   Ed
• Staph transposon   Ed
• Test   Conan Eddy
• PCR of bugs in berries   SG Edwards
• Help with DNA isolation   SG Edwards
• test, please ignore   Sandra Einerhand
• (no subject)   Sandra Einerhand
• anti-rat chromogranin A antibodies   Sandra Einerhand
• test, please ignore   Sandra Einerhand
• looking for articles on PCR specificity   Rubin Eitan
• electroporation of cosmids   Elke
• Bibliography Software Recommendations   Nick Ellison
• mRNA from yeast for ddRT-PCR   Dianne Emslie
• Human breakpoint region Bcl2   Isabelle Ernest
• cell lines help   Isabelle Ernest
• SmaI digest   Hernan Espinoza
• anti-6Xhis antibody - any new info?   Hernan Espinoza
• Streptavidine Magnetic Particles (SMP)   Glibert Fabrice
• pBELO   Glibert Fabrice
• How to stop the Exo III   Glibert Fabrice
• testing   Theo Fahrendorf
• PRIMERS   Patrick HJ Falckh
• Bibliography Software Recommendations   Patrick HJ Falckh
• cDNA molar equations   Patrick HJ Falckh
• RNA EXTRACTION OF (MURINE) ORGANS FOR PCR   Patrick HJ Falckh
• RNA extraction- Phase Lock   Patrick HJ Falckh
• test, ignore   Don Faller
• Costar Snapwells   Don Faller
• Histone Deacetylase Inhibitors   Douglas V. Faller
• His- binding column   Federici
• Statistical Calculation (Help! is my reasoning okay!!)   Federici
• SDS PAGE and phosphorylation   Ross S Feldberg
• Crithidia luciliae kinetoplast. Help!   Rafel Ferragut
• Help: Refolding of His-tagged protein on Ni-NTA.   Francisco Camara Ferreira
• Yeast Smash and Grab: Can I freeze cell pellet?   S. Findley
• Yeast Smash and Grab: Can I freeze cell pellet?   S. Findley
• Question: Liq Scin Counter: Beckmann vs. Packard   Karl Fischer
• rescue of tranfected DNA   Karl Fischer
• running extracts down hi-trap columns...   Karl Fischer
• anti HBcAg antibodies?   Karl Fischer
• HELP!- Protocol for THP-1: again   Daniel Fishman
• PCR Machines - Suggestions?   Brian A. Flannigan
• In situ PCR   David Fogarty
• Tm calculation short and long oligo's   Noel Fong
• Anti HA ab source?   Michael Forte
• ThermoSequenase   John E. Fox
• Primer storage   John E. Fox
• Test, please ignore   Antonio Rodriguez Franco
• Help for electroporation of E.coli starved cells   Taddei Francois
• 260/280 is 1.6 Good enough?   Richard Van Frank
• documentation and analysis of 2-D gels   Richard Van Frank
• [Q] Sonication times for E. coli?   Richard Van Frank
• test - IGNORE   Bart Frank
• Ignore me - I am only a test   Bart Frank
• exons & protein domains   Amie Franklin
• IEF of DNA binding proteins   Amie Franklin
• RNA Oligo supplier???   Mark Freeman
• Subtractive cDNA   Olivier Gandrillon
• RT-PCR PRIMERS SEQUENCE WANTED   Olivier Gandrillon
• Something living on colchicine!   Olivier Gandrillon
• Touchdown pcr   Olivier Gandrillon
• QIAGEN blood kit screwy?   Frederick Garbrecht
• method for extracting DNA and RNA from same sample.   Frederick Garbrecht
• PCR with fluorescein labeled nucleotides   Frederick Garbrecht
• cloning "elongase" PCR products   Frederick Garbrecht
• MDCK/VERO proterties   Mike Garbutt
• Biotin dUTP's   Wes Garrett
• 365nm transilluminator for photodocumentation?   Szatmari George
• 4 way ligation avoided !!!!!!   Gabi Gerlitz
• carbenicillin   Torben Gjetting
• Advice on anti Ras Ab's needed   Ole Gjoerup
• Help with DNA isolation   Michelle Gleeson
• Cutting glass for sequencing plates?   Michelle Gleeson
• Blotting of PFGE   Dr. Richard V. Goering
• How can I inhibit degradation of a dipeptide?   Steven Goldberg
• E.coli and toxic proteins   Steven Goldberg
• protein structure prediction   Josh Goldstein
• Amplitaq gold problems   Michael Gorry
• ABI Chromosome Marker Panels   Michael C. Gorry
• Something living on colchicine!   David Grant
• subcloning pcr bands   Enrique Graziano-Areste
• Stuttering (Laddering) in Sequencing Gel   Roger C. Green
• RT-PCR Problems   S K Green
• help   Olivier Grenet
• Help--Cultured fibroblasts come off coverslips   Brian T. Greuel
• yeast tRNA amber suppressor and John Tesh   GriffCouch
• unbiotinylating streptavidin ... ?   Duke Groebe
• protein domains   Bob Gross
• exons & protein domains   Bob Gross
• exons & protein domains   Bob Gross
• Stutter Bands   David Groth
• pGEM-T cloning   Christoph Grunau
• help for HPLC anionic   Christoph Grunau
• removal of imidazole   Toumy Guettouche
• Antibodies to poly-His   Toumy Guettouche
• GroE chaperonin over expression constructs for recomb. prot. or phage-display, etc.   Joel Guthridge
• MMTV PROMOTER   Ferland Louis H.
• Antibody Technology and Applications Meeting   Bernd Haase
• Wax Beads for PCR   Robert Hamilton
• How do you quantitate DNA with a flourimeter?   Gary Hannan
• Murine fibroblasts   Rizwan Haq
• GFP and Flow cytometry   Rizwan Haq
• *Reliable* RPA method?   Phil Hardy
• Help find K cacodylate source! Thanks!   HaroldD885
• RNA secondary structure predictions - G-U pairing   HaroldD885
• SmaI digest   MV. Hayes
• Qiagen plasmid maxi kit; low yields   MV. Hayes
• VEGF165 cDNA needed   Heinz Rupp, PhD
• Source for VEGF165   Heinz Rupp, PhD
• Streptavidine Magnetic Particles (SMP)   Paul N Hengen
• Lambda Pop6   Paul N Hengen
• PCR of bugs in berries   Paul N Hengen
• Ethidium question   Paul N Hengen
• Quantitative PCR   Paul N Hengen
• Pointer to FAQ lists   Paul N Hengen
• long PCR   Paul N Hengen
• about DNA recovery from agarose gel   Paul N Hengen
• Transposons   Paul N Hengen
• Help: make primers from amino acid sequence   Paul N Hengen
• Taq polymerase patent (not)   Paul N Hengen
• Average molecular weight of a DNA base pair   Paul N Hengen
• Average molecular weight of a DNA base pair   Paul N Hengen
• DNA binding to silica   Paul N Hengen
• Stutter Bands   Paul N Hengen
• Concentration of DNA in Ligations???   Paul N Hengen
• Need assistance re: Alkaline Elution of DNA   Kevin Henne
• Telomeric repeat amplification protocol (Kit?)   David Hieber
• PCR of bugs in berries   Walter Hill
• salt conc. and DNA mobility question   Jody K. Hirsh
• RPA's   Miss. V.M. Hodges
• pGEM-T cloning   Miss. V.M. Hodges
• site directed mutagenesis kits   Miss. V.M. Hodges
• long range PCR of genomic insert in Lambda   Carsten Hoege
• Custom antibody needed   Charlie Hoffman
• Cutting glass for sequencing plates?   Rosemary T. Hoffman
• Help: make primers from amino acid sequence   Rosemary T. Hoffman
• site directed mutagenesis kits   Marcel Hoffmann
• Sonication   Susan Hogarth
• 260/280 is 1.6 Good enough?   Susan Hogarth
• ACTION ALERT Fetal Bovine Serum production.   Susan Jane Hogarth
• DNA quantitation   Susan Jane Hogarth
• PROTEIN DEPLETED FOOD FOR MICE   Susan Jane Hogarth
• Separate ss/ds DNA   Susan Jane Hogarth
• Native PAGE of very basic small protein   Dave Hollingworth
• Imanishi-Kari coverage   Louis Hom
• Primer Problems   Hopkins, Charlotte
• Q-PCR using AmpliSensor methodology   Stephen J. Hopkins
• Fluoresceinated nucleotide-mix   Eivind Hovig
• MJ-Research DNA Engine   Ken Howe
• Tm calculation short and long oligo's   Ken Howe
• exons & protein domains   Ken Howe
• Average molecular weight of a DNA base pair   Ken Howe
• yeast amber suppressor tRNA   Ken Howe
• yeast tRNA amber suppressor and John Tesh   Ken Howe
• Detecting DNA-binding proteins   Emily Howman
• Ni chelate affinity chromatography   Mr. Chris Hoyle
• tobacco cDNA library with GAL4?   Zheng Huanquan
• Nuclear extracts from peripheral blood lymphocytes   Chris Hughes
• weird RNA pattern   Gene Huh
• Dissolving peptides   Trish Hurley
• probe labeling   Carl S. Raimond III
• about DNA recovery from agarose gel   Jin-seon Im
• Custom Antibody Needed   Signature Immunologics
• dem bones   ImmGen Inc.
• Autoclaving SDS ?   Frederik Intelmann
• Thermostable RNA polymerase   Takahiko Ishiguro
• Elastase production   University of Palermo - Italy
• Elastase production   University of Palermo - Italy
• Elastase production   University of Palermo - Italy
• Inosine containing primers   Fraser Dr N J
• MICROEXTRACTION   Fraser Dr N J
• electroporation of cosmids   Fraser Dr N J
• PCR using a primer with a dUTP   Fraser Dr N J
• opinions on pCR-Script (Stratagene)   Fraser Dr N J
• Nigericin help   JAM
• Cloning very large inserts. Help   JCULI at MVAX.CBM.UAM.ES
• probe separation   JLBlackwell
• Four-tracking from Hell; (Taq-sequencing?)   Daniel Jacobs
• Help with DNA isolation   Daniel Jacobs
• plasmid-preparation   Daniel Jacobs
• Rapid hybridization buffer   Nick Jacobsen
• Ethidium question   Nick Jacobsen
• about DNA recovery from agarose gel   Nick Jacobsen
• Bibliography Software Recommendations   James S. Sutcliffe, Ph.D.
• Annealing problems...   Narasimham Jammi
• (no subject)   Geert Jannes
• mRNA stability   Geert Jannes
• test   Raymond B. Jansen
• plasmid-preparation   Raymond B. Jansen
• Wanted: supF bacteria   Glenn Jenkins
• Wanted: supF bacteria   Glenn Jenkins
• Help--Cultured fibroblasts come off coverslips   Dan Johnson
• Concentration of DNA in Ligations???   David R. Johnson
• PCR using a primer with a dUTP   Julie Johnson
• GpppG cap and oocytes?   Jillian Johnston
• Transposon Mutagenesis HELP PLEASE   Mick Jones
• removal of imidazole   Zophonias O. Jonsson
• Ni-NTA/HiTrap-Chelating, urea, the pH, and thiol reagents   Zophonias O. Jonsson
• microinjecting large DNA   Robert Jordan
• Thioredoxin fusion expression?   Fourie Joubert
• DNA binding to silica   Kay Junghanns
• spermidine/betaine/ssbp storage?   Dr. K.Hoare
• ELISA covers for PCR   GERALDINE KAMINSKI
• gunk in RNA extraction   GERALDINE KAMINSKI
• DNA Mass Ladder problems   KARU
• poly A signal   Alan KAY
• Mac scanner for blots...   Alan KAY
• Problems with one base extension cloning.   Alan KAY
• NEB Alkaline phosphatase contain Nuclease?   KHUANG at CHEMVX.TAMU.EDU
• long PCR   KHUANG at CHEMVX.TAMU.EDU
• pGEM-TA cloning   KHUANG at CHEMVX.TAMU.EDU
• Source for KlenTaq   KHUANG at CHEMVX.TAMU.EDU
• Isolation of "hot" oligos   Dr. Dietmar Kahle
• I'm looking for a piece of software that does...   Makoto Kamei
• ice like proteases   Doris Karbach
• Amplitaq gold problems   pe.com/home/netscape-galleria.htmlonnection: Keep-Alive
• C-terminal protein sequencing   Douglas B. Kell
• about DNA recovery from agarose gel   Jill Kellogg
• Primer Problems   Jill Kellogg
• pellet paint interfere with DNA measurement ?   Jill Kellogg
• NEED HELP: Chitinase IEF gel   Gabre Kemp
• Please HELP: Staining Glucose N-Acetyl units   Gabre Kemp
• Something living on colchicine!   Michael Kertesz
• I need a good cosmid isolation protocol   Michael Kertesz
• Touchdown pcr   Michael Kertesz
• Detection of phosphorylation in non-denaturing condition   Dooyeon Kim
• PCR genotyping   Dr. T.F. King
• I need a good cosmid isolation protocol   Michael R. King
• Question: Liq Scin Counter: Beckmann vs. Packard   Dima Klenchin
• % of ammonium sulphate - PKC precipitation?   Dima Klenchin
• anti-rat chromogranin A antibodies   Dima Klenchin
• electroporation of cosmids   Dima Klenchin
• Sure way to quantify Northern blots   Dima Klenchin
• unbiotinylating streptavidin ... ?   Dima Klenchin
• unbiotinylating streptavidin ... ?   Dima Klenchin
• Wanted: buffer for pH 2.5-3!!   Dima Klenchin
• Anti HA ab source?   Dima Klenchin
• Wizard preps for "advanced" uses   Dima Klenchin
• Ni-NTA/HiTrap-Chelating, urea, the pH, and thiol reagents   Dima Klenchin
• Something living on colchicine!   Dima Klenchin
• Transfecting A549 cells   Dima Klenchin
• Qiagen DNA purification resin - what is is?   Dima Klenchin
• SDS PAGE and phosphorylation   Dima Klenchin
• DNA purification   Michael Kloth
• Library of E. coli   Riekeltje Koedood
• Blue color comes late lately in yeast 2 hybrid   Riekeltje Koedood
• Blue color comes late lately in yeast 2 hybrid   Riekeltje Koedood
• Blue color comes late lately in yeast 2 hybrid   Riekeltje Koedood
• Vacuum dot blotter for 96 well plates ???   Piotr Kozbial
• Cutting glass for sequencing plates?   Alexander Kraev
• how many bacteria in a colony   Candace Krepel
• High background with SDS   Jens Krieger
• I need a good cosmid isolation protocol   David Kristofferson
• Zeocin Problems   Georg Kroeger
• Average molecular weight of a DNA base pair   Dr. Andrew Kropinski
• Gene 32 and PCR   Warren D. Kruger
• RPA's   Gary Kucera
• *Reliable* RPA method?   Gary Kucera
• MJ Research PCR machines: Any Happy Customers?   Gary Kucera
• SSCP: Restriction Enzymes vs. Shorter Fragments???   Chris Kyle
• Cohesive End Ligations - Question   Kathy L
• Cohesive End Ligations - Question   Kathy L
• I need a good cosmid isolation protocol   martin LEACH
• Vacuum dot blotter for 96 well plates ???   martin LEACH
• isolation of DNA from mouse tails   martin LEACH
• gradient pcr?   martin LEACH
• gradient pcr?   martin LEACH
• European Radiochemical suppliers   martin LEACH
• Comparing products Magazine   martin LEACH
• RT-PCR Problems   John Ladasky
• SmaI digest   Jean-Francois Larrivee
• method for extracting DNA and RNA from same sample.   Jean-Francois Larrivee
• Sure way to quantify Northern blots   Jean-Francois Larrivee
• DEAE transfection   Jean-Francois Larrivee
• Molecular biology software search   Dominique Larrouy
• Proofreading DNA polymerases and genomic DNA   Flemming Larsen
• Q: cheaper sequencing plates for ABI 373A?   Michael Lassner
• DEPC   Edouard Lauzier
• DIG question...   Edouard Lauzier
• NIH Image advice...   Edouard Lauzier
• Source for Aminoallyl-dUTP   LeeSylvers
• DNA Extraction From Agarose   LeeSylvers
• unbiotinylating streptavidin ... ?   LeeSylvers
• unbiotinylating streptavidin ... ?   LeeSylvers
• unbiotinylating streptavidin ... ?   LeeSylvers
• unbiotinylating streptavidin ... ?   LeeSylvers
• Kits for H. pylori   LeeSylvers
• DEAE transfection   Philippe Lefebvre
• Touchdown pcr   Philippe Lefebvre
• Centimorgans vs Lenght in kb   Rachelle Leger
• test, please ignore   Doriana Lehmann
• HELP: pcr-problems with EUROGENTEC   Doriana Lehmann
• thank you   Doriana Lehmann
• Tm calculation   Marc Lemaître
• c-Myc Cassettes   Alfredo Lopez De Leon
• Which determines mobility?   Keith Leppard
• ScFv expression in pVIII-coat protein??????   Danny T M Leung
• IRES-Element   Victor Levenson
• Looking for SV40 tsA58 or tsA239 expression vector   Victor Levenson
• How to specifically eliminate cilia (or beating)?   Michael Levin
• plaque hybridization using non-radioactive labled probes   Paul & Orit Levius
• Help with PCR library screening   Dr. Miguel A. Gomez Lim
• formalin extractions   Henning Lindeberg
• Blotting of PFGE   Julia Lodge
• Propagating errors   Michael Lonetto
• beta gal assay   Ray Lu
• beta gal assay   Ray Lu
• E.coli and toxic proteins   Jennifer Lyon
• Dot blot software   TERRY MACINTYRE
• Ph.D. Position available   HAUSER MARIE-THERES
• Bubble-getters   IAN MCFARLANE
• Problems with lipids   IAN MCFARLANE
• NIH Image advice...   Paul C. Grimm MD
• Primer Problems   MELANSOND
• probe separation   MELANSOND
• Depc water   MELANSOND
• How do you quantitate DNA with a flourimeter?   MELANSOND
• geneclean: vortexing   MELANSOND
• How to "normalize"   MELANSOND
• Commenting from file   MILLER at HELIX.MGH.HARVARD.EDU
• genomic mouse clones   FABIAN MOEBIUS
• Rat TRF?   MONSTER
• Size markers for sequencing gel   Zhengqiang Ma
• Ab against P-ser, P-thr and P-tyr   Joe Maa
• site directed mutagenesis kits   M. MacDonald
• blue colonies-no Xgal   Rafael Maldonado
• P1 transductions   Rafael Maldonado
• NEB Alkaline phosphatase contain Nuclease?   Rafael Maldonado
• Blue color comes late lately in yeast 2 hybrid   Rafael Maldonado
• Something living on colchicine!   Rafael Maldonado
• method for extracting DNA and RNA from same sample.   Terese Maltzman
• failproof rt-pcr kit   Terese Maltzman
• Cohesive End Ligations - Question   Ned Mantei
• Autoclaving SDS ?   Ned Mantei
• Sequencing gels   Ned Mantei
• Concentration of DNA in Ligations???   Ned Mantei
• salt conc. and DNA mobility question   Ned Mantei
• [Q] Sonication times for E. coli?   Ned Mantei
• GFP users in UK   Jose F Gutierrez Marcos
• Commercial sequencing costs????   Jeremy Marcus
• formalin extractions   Peter Marko
• !!!!!! Antibody against tissue factor !!!!!!   David Martin
• four-way ligation help !!!   David Martin
• Is anyone using 33P in RNase protection assays/Solution hybridization?   Don Martinson
• Ethidium question   Don Martinson
• isolation of DNA from mouse tails   Matthew P. Frosch, M.D., Ph.D.
• MJ-Research DNA Engine   George F. Mayhew
• opinions on pCR-Script (Stratagene)   Peter McClive
• Service contracts for ABI Automated DNA Sequencer   Johnjoe McFadden
• purity of urea for sequencing gels   Ruth McMahon
• isolation of DNA from mouse tails   Betty-anne Mcbey
• HIV genomic RNA: polyA?   Md1214
• Expression vectors for microsomal protein   Meetha Medhora
• Non-isotopic sequencing   Gerardo Medrano
• dephosphorylation by SAP   Ian J. Mehr
• c-myb   Harutyan Melkonyan
• footprint   Harutyan Melkonyan
• DNA-quantification   Harutyan Melkonyan
• unsubscribe   Tom Menees
• high guluronic acid alginate   Thomas J. Merchant
• post-doc positions in plant molecular biology   Thomas J. Merchant
• DNA ligation   David Micklem
• SmaI digest   Mark R. Miglarese
• Bibliography Software Recommendations   Andrew Millar
• Source for Anti-Betalactamase?   Charles A Miller
• How do you quantitate DNA with a flourimeter?   Charles A Miller
• DNA quantitation   Lisa M Miller
• I need an expression vector with amplfiable selective marker   David Misztal
• GpppG cap and oocytes?   Laboratorio de Fisiopatologia Molecular
• Fluorescent Labelled Chemokines.   Chris Moore
• Something living on colchicine!   Grant Morahan
• backgroound problems with Hybond N   David D Morgan
• Suppression pcr - what is it?   Stephen Morris
• probe separation   Thibaud Le Mouel
• Using ClaI for subcloning   Scott Moye-Rowley
• RT-PCR Problems   Paul Mozdziak
• Photoprotein obelin as a marker for cell-free proteinbosynthese   Mtberlin
• rescue of tranfected DNA   Ben Mulder
• Alpha-complementation in mammalian cells?   Ben Murray
• RNASE DECONTAMINATION REVIEW (apology)   Bernard Murray
• Four-tracking from Hell; (Taq-sequencing?)   Daniel Mytelka
• tips and tricks for lambda genomic library construction?   Jon Nakamoto
• Research Scientist- Molecular Biology-Signal Transduction   Krishnan Nandabalan
• Gibco/BRL's web adress   Rick Neubig
• Source for EF-G ?   John A. Newitt
• Lambda Ziplox   Thomas Newman
• Wizard preps for "advanced" uses   Chia Jin Ngee
• Genie DNA System (was Wizard preps for "advanced" uses)   Chia Jin Ngee
• Concentration of DNA in Ligations???   Chia Jin Ngee
• recommendations for microfuge?   Chia Jin Ngee
• triphenyltetrazolium chloride agar   Toai T. Nguyen
• looking for BJ5183 E.Coli   Brad Nicholson
• two-hybrid cloning systems   Rick Nicholson
• MJ-Research DNA Engine   Rick Nicholson
• method for extracting DNA and RNA from same sample.   Rick Nicholson
• MJ-Research DNA Engine   Rick Nicholson
• Primer Problems   Rick Nicholson
• SmaI digest   Michele Normore
• DNA quantitation   Pamela Norton
• *Reliable* RPA method?   Pamela Norton
• Using ClaI for subcloning   Pamela Norton
• TRIZOL   Lauryl Maiya J Nutter
• Glycin/Hcl   Martin Offterdinger
• Northern with DIG   Martin Offterdinger
• quantitative PCR   Martin Offterdinger
• viable western blots   Martin Offterdinger
• DIG band shifts   Martin Offterdinger
• Library of E. coli   Namy Olivier
• Yeast Smash and Grab: Can I freeze cell pellet?   Namy Olivier
• PCR Mimic   Myriam Onno
• protein sequencing   Myriam Onno
• cDNA characterization using antibodies   Myriam Onno
• Search for germinal cell lines   Myriam Onno
• Subtractive cDNA   Sandra Pena De Ortiz
• method for extracting DNA and RNA from same sample.   Sandra Pena De Ortiz
• Gene Sequencing   Sandra Pena De Ortiz
• Subtractive cDNA   Sandra Pena De Ortiz
• Gene Sequencing   Sandra Pena De Ortiz
• Subtractive cDNA   Sandra Pena De Ortiz
• Nested Deletion Kit   Sandra Pena De Ortiz
• tubes for in-situ hybridization   Sandra Pena De Ortiz
• Subtractive cDNA Hybridization:   Sandra Pena De Ortiz
• Antibody against phosphoserine/threonine   Stephane Ory
• Cytochalasin B or cytochalasin D ?   Stephane Ory
• Custom antibody needed   Guy Oshiro
• Ni-NTA/HiTrap-Chelating, urea, the pH, and thiol reagents   J.M. Ossewaarde
• Phosphohistidine/Phosphoaspartate Antibodies   Darin B. Ostrander
• Dig-riboprobes and RNase A   Kerstin Otte
• Sonication Protocol!!   Brian J. Paeng
• MJ-Research DNA Engine   Soonmyoung Paik
• Subtractive cDNA   Soonmyoung Paik
• GFP stability in fixed tissues   Mark S. Parcells
• Non-radioactive colony hybridization   Yoonsuk Park
• Bibliography Software Recommendations   Yoonsuk Park
• Imanishi-Kari coverage   David C. Parker
• 13C source for protein NMR   Matthew Parker
• Monoclonal Antibody databases   Mick Partis
• how many bacteria in a colony   Pascal_Bochet
• Lac operon curing?   Doug Pecota
• Need Transfection Protocol   Andrea Marie Peier
• Tm calculation short and long oligo's   Richard Pelletier
• ELISA covers for PCR   Pete
• (none)   Karel Petrzik
• what is strong stop-DNA?   Robert Phelps
• ERRATUM: PFGE to separate 20-50 kb   Andy Phillips
• Is KlenTaq commercially available?   Robert Phillips
• Gibco/BRL's web adress   Markus Piotrowski
• 32P-probe by PCR?   Markus Piotrowski
• I'm looking for a piece of software that does...   Markus Piotrowski
• Something living on colchicine!   M. Plewinska
• Locus-Pocus   Jan van der Ploeg
• wanted: gal4 luciferase reporter   Scott Vande Pol
• mutant neo gene for targeting   Andrei Popov
• Something living on colchicine!   Andrei Popov
• ERRATUM: PFGE to separate 20-50 kb   Andrei Popov
• Blotting of PFGE   Andrei Popov
• PolyA column   Karen Posey
• QuikChange=QuickWaste?   Postmaster at p001578.icos.com
• Depc water   Douglas Prasher
• probe separation   Johanna Preiszner
• anti-6Xhis antibody - any new info?   Mark Prescott
• gradient pcr?   Kiley R. Prilliman
• Cosmid purfication with QIAGEN minipreps   QIAGEN
• Help with DNA isolation   R.Peeters at nunhems.nl
• PRIMERS   RBLLC at CUNYVM.CUNY.EDU
• Rice cell cryopreservation   REGCHAK at USTHK.UST.HK
• PCR problem   REGCHAK at USTHK.UST.HK
• Adding 3' terminal thymidine to blunt ended DNA   Simon Q.J. RICE
• MIPTEC 96   RManns7
• A with a flourimeter?   RManns7
• Costar Snapwells   RManns7
• Comparing products Magazine   RManns7
• dephosphorylation by SAP   H. ROYCHOWDHURY
• probe separation   H. ROYCHOWDHURY
• Depc water   H. ROYCHOWDHURY
• Separate ss/ds DNA   H. ROYCHOWDHURY
• Concentration of DNA in Ligations???   H. ROYCHOWDHURY
• Size markers for sequencing gel   H. ROYCHOWDHURY
• Staining gels in a microwave   H. ROYCHOWDHURY
• RNA through the years...   H. ROYCHOWDHURY
• baculo TCR expression   Christian Radauer
• In search of a way to compare nubcleotide sequences with GenBank data.   Carl Raimond
• M13 Bacteriophage and Glucose   Keith Rand
• Lac operon curing?   Keith Rand
• Qiagen plasmid maxi kit; low yields   Miguel Rea
• Glycin/Hcl   Achim Recktenwald
• DNA-Elution from gel   Reinecke
• Beckman JLA-10.5 rotor problems?   S. James Remington
• Looking for H. Bujard's Tet system plasmids: pBI-4 ...   Eric Rheaume
• Gene II   David M Rivers
• RNA blot question   Julie Roberts
• Transfecting A549 cells   Robin Ann Wasserman, Ph.D.
• % of ammonium sulphate - PKC precipitation?   Phil Robinson
• Detection of phosphorylation in non-denaturing condition   Phil Robinson
• Help: make primers from amino acid sequence   Phil Robinson
• quantification of low concentrations of DNA   Miriam Rosin
• quantification of low concentrations of DNA (fwd)   Miriam Rosin
• about DNA recovery from agarose gel   Eitan Rubin
• Taq polymerase patent (not)   Eitan Rubin
• Four-tracking from Hell; (Taq-sequencing?)   Dr. A. Ryan
• Human DNA quantification   Filip Ryniers
• Help for DNA quantification   Filip Ryniers
• in situs with oligos?   S.K.Green
• Muscular dystrophy: A RANDOMIZED TRIAL OF BEMITHYL IN MUSCULAR DYSTROPHIES   STEMC
• shell-less CAM assay   Rene Mauricio Salazar
• viable western blots   Rene Mauricio Salazar
• DNA translation   Klaus Salger
• In search of a way to compare nubcleotide sequences with GenBank data.   Klaus Salger
• 4 way ligation avoided !!!!!!   Klaus Salger
• probe separation   Klaus Salger
• luciferase N-terminal deletion   Klaus Salger
• Northern with DIG   Klaus Salger
• Bootscanning for recombination   Mika Salminen
• Bootscanning NOTICE!   Mika Salminen
• (no subject)   Babru Samal
• The structure of animal genes and genomes   Phillip SanMiguel
• Gel Shift assay   Heinz-Juergen Schaefers
• REQ: EMSA (gelshift) protocol   Heinz-Juergen Schaefers
• Transcription factor binding sites   Heinz-Juergen Schaefers
• EMSA-protocol: Additions and error correction   Heinz-Juergen Schaefers
• Primer Problems   Heinz-Juergen Schaefers
• RNA blot question   Heinz-Juergen Schaefers
• RNA blot question   Heinz-Juergen Schaefers
• How to "normalize"   Heinz-Juergen Schaefers
• SWISSPROT update   Denni Schnapp
• Mac Scanner   Denni Schnapp
• enzyme inhibition   Denni Schnapp
• PAGE of basic proteins   Denni Schnapp
• New FDA Regs   Phillip E. Schwartz
• unsubscribe   Tanja Schwuchow
• Poor RNA yield from mouse pancreas   Andrew C Scott
• Molecular Modelling   Vikas Shah
• Ericomp Thermal Cycler   Michal Shapira
• Ericomp Thermal Cycler   Michal Shapira
• Ericomp Thermal Cycler   Michal Shapira
• Ericomp Thermal Cycler   Michal Shapira
• Seeking source of pipette tips   Hr Dr. S. Shapiro
• isolation of DNA from mouse tails   John J. Shaskus
• How do you quantitate DNA with a flourimeter?   John J. Shaskus
• PAI myeloma cell line and pseudogenes   Regina Shaw
• ? about DNA extraction kits   benedicte Shevchenko
• updated WWWsite: The Antibody Resource Page   Kevin Shreder
• [Q] Sonication times for E. coli?   Timur Shtatland
• Degenerative PCR-primers - high GC content   Eirikur Sigurdsson
• Detction of biotinylated oligos   Skip
• RNA from Arabsidopsis   Mike Sklar
• Primer Sequences   Mike Sklar
• IRES-Element   Emmanuel Skoufos
• long PCR?   Kimberley Snowden
• Uniequip DNA Workstation - comments?   Pam Snyder
• ELISA covers for PCR   Keld Sorensen
• Methods & Reagents Newsgroups   Keld Sorensen
• Rapid hybridization buffer   Keld Sorensen
• Streptavidine Magnetic Particles (SMP)   Keld Sorensen
• Use of RIA human insulin with ovine plasma   Keld Sorensen
• unbiotinylating streptavidin ... ?   Keld Sorensen
• Roche did not get patent on naturally occurring Taq!   Keld Sorensen
• unbiotinylating streptavidin ... ?   Keld Sorensen
• Dot blot software   Keld Sorensen
• MIPTEC 96 (Microplate Conference)   Keld Sorensen
• Wanted: buffer for pH 2.5-3!!   Keld Sorensen
• recommendations for microfuge?   Keld Sorensen
• Vacuum dot blotter for 96 well plates ???   Keld Sorensen
• Wax Beads for PCR   Keld Sorensen
• Thermocyclers   Keld Sorensen
• Costar Snapwells   Keld Sorensen
• Comparing products Magazine   Keld Sorensen
• QIAGEN MINIPREP ROBOT   Augustine Souza
• GST-fusion interaction--high background??   Pavel Sova
• reference for "Merlin" prep method   J. David Spafford
• backgroound problems with Hybond N   J. David Spafford
• T cell receptor PCR   Silvia Spitzer
• Human cDNA library in yeast expression vector   Guillaume Stahl
• Using ClaI for subcloning   John Stebbins
• exons & protein domains   Arlin Stoltzfus
• Xenopus borealis cDNA library   Gareth Sullivan
• Xenopus borealis cDNA library   Gareth Sullivan
• silver staining...   Sailesh Surapureddi
• denaturing dna phastgels + silver staining of same   Dai Suter
• SmaI digest   Steven Tait
• BRL Random Priming   Steven Tait
• Green Bacteria Cultures   Steven Tait
• protein structure prediction   Seng-Lai Tan
• GST-fusion interaction--high background??   Seng-Lai Tan
• cross-linking protein kinase   Seng-Lai Tan
• Bubble getter   Agnes Tay
• Taq polymerase patent (not)   Dr G R Taylor
• Amplitaq gold problems   Dr G R Taylor
• PCR and seq. of microsat. region   Dr G R Taylor
• anti HBcAg antibodies?   Graham Taylor
• Something living on colchicine!   Chromosome Terror
• Something living on colchicine!   Chromosome Terror
• isolation of DNA from mouse tails   Tom Thatcher
• Depc water   Thomas
• BRL Random Priming   Michael W. Thompson
• How can I inhibit degradation of a dipeptide?   Michael W. Thompson
• How can I inhibit degradation of a dipeptide?   Michael W. Thompson
• How can I inhibit degradation of a dipeptide?   Michael W. Thompson
• Something living on colchicine!   Michael W. Thompson
• subcloning pcr bands   Michael W. Thompson
• Molecular biology software search   Michael W. Thompson
• N-terminal His-tag expression vector   Michael W. Thompson
• Ethidium question   Rick Thorne
• IEF of DNA binding proteins   Laurence Tiley
• RNA EXTRACTION OF (MURINE) ORGANS FOR PCR   Ulrich Tillmann
• isolation of DNA from mouse tails   Ulrich Tillmann
• Custom oligosynthesis and custom protein sequencing   Anthony Tomlinson
• ThermoSequenase   Anthony Tomlinson
• Cutting glass for sequencing plates?   Roopie Toops
• Tm calculation short and long oligo's   Tori
• Chlorella culture media   Jorge Hernandez Torres
• Methylene Blue and ssDNA   Joe M Trakalo
• Simple helper software   Marek Treiman
• Carbon 14 Assay Instrument   Rich Treinen
• Assay for Carbon-14   Richard J Treinen
• Propagating errors   Gerard Tromp
• Primer Design???   Chris Troy
• pGEM-T cloning   John Troyer
• DEAE transfection   John Troyer
• I need a good cosmid isolation protocol   John Troyer
• Karen Browning?   Gilles Truan
• Ficoll removal from organelle lysates   Tung Tran Duc Tuan
• HOW WIDE IS ANTIBODY'S BITE?   Michael J. Tuvim
• pSCAN8   Antoine VEKRIS
• Help regarding availability of Mwo I restriction enzyme   Subramaniam Vadakanthara
• Antibodies to poly-His   A. Vairis
• Computational Molecular Biology Workshop, October 15-19, 1996   Iosif Vaisman
• ligation help!!   Edgar Valencia
• need mouse placental cell line   VanHouten, Joshua
• I need an expression vector with amplfiable selective marker   Tom Vink
• endotoxin kit   Marc Visconti
• Lambda Ziplox   Botma Visser
• dephosphorylation by SAP   Dr A.H.M. Van Vliet
• Staining gels in a microwave   Dr A.H.M. Van Vliet
• long oligonucleotides   Matthias Vogel
• IRES-Element   Matthias Vogel
• QE vectors   Matthias Vogel
• Thermostable eukaryotes DNA libraries   Karl Voss
• ThermoSequenase   Karl Voss
• PCR and seq. of microsat. region   C.S. WILDING
• Kits for H. pylori   SURASAK WONGRATANACHEEWIN
• Rapid hybridization buffer   JOHNNY WOOD
• Elisa Covers, Films for PCR plates   WSchick at aol.com
• Repair of MJ Resrch PTC-100 (PCR machine)   WSchick at aol.com
• documentation and analysis of 2-D gels   WSchick at aol.com
• Touchdown pcr   WSchick at aol.com
• Amplitaq gold problems   WSchick at aol.com
• recommendations for microfuge?   WSchick at aol.com
• Blotting of PFGE   WSchick at aol.com
• QPCR with ABI System 7700   WSchick at aol.com
• Proofreading DNA polymerases and genomic DNA   WSchick at aol.com
• Cohesive End Ligations - Question   WUSC
• recommendations for microfuge?   Paul Wakenight
• Tm calculation short and long oligo's   Mark Walberg
• Bibliography Software Recommendations   Andrew Wallace
• RT-PCR Problems   Edward Wang
• Klenow and Agarose...   Edward Wang
• Q: cheaper sequencing plates for ABI 373A?   Peter Wang
• AFLP Problems   Yihong Wang
• Stuttering (Laddering) in Sequencing Gel   Yihong Wang
• SmaI digest   John Watson
• 13C source for protein NMR   Christoph Weber
• Tm calculation short and long oligo's   Ke Wei
• Help--Cultured fibroblasts come off coverslips   Ke Wei
• about DNA recovery from agarose gel   Ke Wei
• HELP: electroblot a Coomasie Stained PAG onto a Membrane   Ke Wei
• It is possible to get RNA from paraffin blocks?   Ke Wei
• semi-dry southern blotting   Coen van der Weijden
• Elisa for Q-RT-PCR   Franz Weilbach
• PCR Machines - Suggestions?   Jory Weintraub
• Statistical Calculation (Help! is my reasoning okay!!)   Chi-Kuang Wen
• TRIZOL   Chi-kuang Wen
• Adding 3' terminal thymidine to blunt ended DNA   Chi-kuang Wen
• Subtractive cDNA   Rong Wen
• Rapid hybridization buffer   Mark S. Whitsitt
• footprint   Dieter Wiesmann
• DNA-quantification   Dieter Wiesmann
• DNA-quantification by software   Dieter Wiesmann
• Desperately seeking DrdI   Deborah Wilkinson
• pWE15cosmid library   Deborah Wilkinson
• looking for BJ5183 cells   William
• looking for BJ5183 E.Coli   William
• Antisense Protocols   William_P_Prichett
• GibcoBRL/Lifetechnologies   Ann Williams
• Cohesive End Ligations - Question   Ann Williams
• 13C source for protein NMR   Randall Willis
• 13C source for protein NMR   Randall Willis
• c-Myc Cassettes   Troels Wind
• Advice on anti Ras Ab's needed   Troels Wind
• Advice on anti Ras Ab's needed   Troels Wind
• Cytochalasin B or cytochalasin D ?   Herbert H. Winkler
• His- binding column   Herbert H. Winkler
• Bibliography Software Recommendations   Herbert H. Winkler
• Assay for Carbon-14   Herbert H. Winkler
• IgM digestion?   Christoph H. Winter
• how many bacteria in a colony   Christoph H. Winter
• Phosphorylation protocol   Christoph H. Winter
• probe separation   David Winterbourne
• need (aryl)sulfatase cDNA   Ute Wittstock
• sorry, it doesn't work   Ute Wittstock
• test   Ute Wittstock
• planet planaria   Arthur Wohlwill
• Help: PCR problems with D12S99 Just wondering if anyone else has been having problems with their primer set for the D12S99 locus (12p13 region). We have been working with the probe AFM217xa7a/AFM217xa7m and have had no success, after having tried all of the following conditions: Annealing temp: 50, 55, 60 Initial Melting temp: 92, 94 Mg2+ conc: 1.5 - 4.0 mM Primer conc: 1X, 3X Glycerol/no glycerol Any more suggestions??? Thanks!!   John Wolff
• Antibody against phosphoserine/threonine   Jim Woodgett
• Mac scanner for blots...   Jim Woodgett
• Phosphorylation protocol   Jim Woodgett
• SDS PAGE and phosphorylation   Jim Woodgett
• yeast two hybrid system   R. Woodward
• Something living on colchicine!   Russell Wrobel
• Expression of toxic proteins:   Michael Yacuboski
• help:Mac based gel Documentation systems   Chihiro Yamada
• pellet paint interfere with DNA measurement ?   Ziping Yang
• pGEM-T cloning   Minsoo Yoon
• DNA translation   Pak-Lam Yu
• What's the best RACE system?   Dale Yuzuki
• Yeast genome data bank access   Matthias Zeiner
• GST-fusion interaction--high background??   Matthias Zeiner
• Help! How to stop them?   Zhiyong Zhao
• gunk in RNA extraction   Zhiyong Zhao
• probe separation   Zhiyong Zhao
• RNA extraction- Phase Lock   Theresa Zwingman
• Mac scanner for blots...   a.f.hill at ic.ac.uk
• PROTEIN DEPLETED FOOD FOR MICE   alena at scientia.up.ac.za
• RNA EXTRACTION OF (MURINE) ORGANS FOR PCR   alena at scientia.up.ac.za
• RT-PCR Problems   gordon allison
• PCR Mimic   gordon allison
• MMTV PROMOTER   annalise at medmicro.uct.ac.za
• Tranfection of fish cells ?   b06g at unb.ca
• Test   baba at osk.threewebnet.or.jp
• Test   baba at osk.threewebnet.or.jp
• documentation and analysis of 2-D gels   michael bausher
• STAT antibodies needed   books at mcvax2.d48.lilly.com
• Primer Problems   brett
• ligation help!!   luis brieba
• Ethidium question   luis brieba
• Gel Shift assay   campa002 at mc.duke.edu
• Tm calculation short and long oligo's   campa002 at mc.duke.edu
• HELP: electroblot a Coomasie Stained PAG onto a Membrane   campa002 at mc.duke.edu
• DIG Northerns   chriso at dfrc.wisc.edu
• RNA Extraction... ( Still )   chriso at dfrc.wisc.edu
• DNA oligo problem..........   chriso at dfrc.wisc.edu
• Northern with DIG   chriso at dfrc.wisc.edu
• backgroound problems with Hybond N   chriso at dfrc.wisc.edu
• Looking for Massimo ZEVIANI   Mike Morris sur cmusun12
• Any new PCR techniques out there   colleenm at s-and-s.com
• Test   dana at zena.uokhsc.edu
• MICROEXTRACTION   dlevitzke
• Protooncogenes expression- cDNA versus RNA Probes   orlando dominguez
• TRIZOL   orlando dominguez
• adaptors: 5' blunt-end phosphorylated?   orlando dominguez
• adaptors: 5' blunt-end phosphorilated?   orlando dominguez
• Adaptors: 5' blunt-end phosphorylated?   orlando dominguez
• subcloning pcr bands   orlando dominguez
• Quantitative PCR   doug.perkins at mcmail.vanderbilt.edu
• temperature induction of BACTERIA   mccaw douglas
• Question: Liq Scin Counter: Beckmann vs. Packard   drdad at ktc.com
• E.coli and toxic proteins   gc
• chloramphenicol concentration   gc
• Tm calculation short and long oligo's   kevin gilbride
• (none)   glycotech
• Detergents   heme at biogen.wblab.lu.se
• Anatrace   heme at biogen.wblab.lu.se
• Ethidium question   Administrador de iihema.fmed.uba.ar
• Is KlenTaq commercially available?   Administrador de iihema.fmed.uba.ar
• BioRads InstaGene Matrix -- How does it work?   Administrador de iihema.fmed.uba.ar
• Thioredoxin fusion expression?   ijiwaru at wheel.dcn.davis.ca.us
• Expression of toxic proteins:   ijiwaru at wheel.dcn.davis.ca.us
• Sequencing gels   immu1
• Gel Shift assay   jackiec at staff.monash.edu.au
• removal of imidazole   jackiec at staff.monash.edu.au
• planet planaria   jgluz at STUD.MED.CORNELL.EDU
• Charon 4A Sequence   jjbinder at students.wisc.edu
• PCR's which produce DNA concatermers   jsowden at uwa.edu.au
• Non-radioactive Tyr-kinase assays?   jstrassw at opal.tufts.edu
• BioRads InstaGene Matrix -- How does it work?   l14y at unb.ca
• How do you convert from Lf/ml to g/ml   l14y at unb.ca
• HELP! Can't reproduce results.   Majumder lab
• How to "normalize"   lmrc.
• How to "normalize"   lmrc.
• In situ PCR   lonnie
• Coefficient of drag for DNA   lonnie
• Transfection of pulmonary artery smooth muscle cells with an adenovirus vector   mahayden at med.unc.edu
• Transfection of pulmonary artery smooth muscle cells with an adenovirus vector   mahayden at med.unc.edu
• RPA's   mahayden at med.unc.edu
• temperature induction of BACTERIA   marcvdc at lmb1.rug.ac.be
• temperature induction in BACTERIA   marcvdc at lmb1.rug.ac.be
• phylogenetic trees   marcvdc at lmb1.rug.ac.be
• help for HPLC anionic   mazella at naxos.unice.fr
• poly A signal   medwards51 at gnn.com
• PRINS, human DNA sequences, reiteration   msms at on-luebeck.de
• Rifampicin activity   nico at lmb1.rug.ac.be
• Help with DNA isolation   nil
• E.coli and toxic proteins   I will not buy your product
• PCR with fluorescein labeled nucleotides   rlee
• whole zebrafish cDNA library   rubind at helix.mgh.harvard.edu
• Control plasmid for GAPDH quantitative RT-PCR   sgibbons at opal.tufts.edu
• N-terminal His-tag expression vector   sherif at DARWIN.UCSC.EDU
• custom protein expression   ssieben at imb-jena.de
• Wizard preps for "advanced" uses   jane staunton
• animal and human cells - vascular and cutaneous   tedsmith at halcyon.com
• TRIZOL   thobman at anat.med.ualberta.ca
• TRIZOL   thobman at anat.med.ualberta.ca
• Amplitaq gold problems   timmer at physio.emory.edu
• jnf test do not read   u.381 at inserm.u-strasbg.fr
• DNA ligation   u.381 at inserm.u-strasbg.fr
• His- binding column   user unknown
• pGEM-T cloning   user unknown
• "Best" Long PCR   varyc.mmcwp4 at mmc.org
• Ligation with two base fill-in   vcore
• IRES-Element   vcore
• Something living on colchicine!   vilimf01 at mcrcr6.med.nyu.edu
• subcloning pcr bands   vilimf01 at mcrcr6.med.nyu.edu
• Proofreading DNA polymerases and genomic DNA   vilimf01 at mcrcr6.med.nyu.edu
• European Radiochemical suppliers   vilimf01 at mcrcr6.med.nyu.edu
• pellet paint interfere with DNA measurement ?   wick at cris.com
• Custom oligosynthesis and custom protein sequencing   Dr.surasakdi wongratanacheewin
• mutant neo gene for targeting   d.m. wright
• DNA ligation   killer yeast
• dephosphorylation by SAP   killer yeast
• yeast two hybrid system   killer yeast
• about DNA recovery from agarose gel   killer yeast
• DNA ligation   killer yeast
• exons & protein domains   killer yeast
• Primer Problems   killer yeast
• DNA ligation   killer yeast
• Something living on colchicine!   killer yeast
• In search of a way to compare nubcleotide sequences with GenBank data.   yewks at teleview.com.sg
• dicistronic vector   lan zhao
• sequence info of pCMV,pCMVrev   lan zhao
• RT-PCR PRIMERS SEQUENCE WANTED   panos zikos
• competitor sequences   panos zikos

Last message date: Wed Jul 31 23:42:19 EST 1996
Archived on: Sun Mar 6 21:06:04 EST 2005

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