Methods July 1996 Archives by date
Starting: Mon Jul 1 02:21:43 EST 1996
Ending: Wed Jul 31 23:42:19 EST 1996
Messages: 1067
- Nuclear extracts from peripheral blood lymphocytes
Kristin B. Andersson
- Monoclonal Antibody databases
Mick Partis
- in situs with oligos?
S.K.Green
- ThermoSequenase
John E. Fox
- Primer storage
John E. Fox
- In situ PCR
David Fogarty
- Question: Liq Scin Counter: Beckmann vs. Packard
drdad at ktc.com
- !!!!!! Antibody against tissue factor !!!!!!
David Martin
- Human DNA quantification
Filip Ryniers
- Oxalyticase in the UK?
Fergus Doherty
- DNA translation
Klaus Salger
- Antibody against phosphoserine/threonine
Stephane Ory
- Question: Liq Scin Counter: Beckmann vs. Packard
Dima Klenchin
- DIG Northerns
chriso at dfrc.wisc.edu
- RNASE DECONTAMINATION REVIEW (apology)
Bernard Murray
- Histone Deacetylase Inhibitors
Douglas V. Faller
- c-Myc Cassettes
Alfredo Lopez De Leon
- Antibody against phosphoserine/threonine
Jim Woodgett
- Expression vectors for microsomal protein
Meetha Medhora
- DNA translation
Pak-Lam Yu
- Beckman JLA-10.5 rotor problems?
S. James Remington
- blue colonies-no Xgal
Rafael Maldonado
- (no subject)
Babru Samal
- two-hybrid cloning systems
Rick Nicholson
- Nuclear extracts from peripheral blood lymphocytes
Chris Hughes
- MJ-Research DNA Engine
Soonmyoung Paik
- poly A signal
medwards51 at gnn.com
- Question: Liq Scin Counter: Beckmann vs. Packard
Karl Fischer
- Help find K cacodylate source! Thanks!
HaroldD885
- RNA secondary structure predictions - G-U pairing
HaroldD885
- help
Olivier Grenet
- Four-tracking from Hell; (Taq-sequencing?)
Dr. A. Ryan
- poly A signal
Alan KAY
- ThermoSequenase
Dr. Duncan Clark
- Subtractive cDNA
Olivier Gandrillon
- Mac scanner for blots...
Alan KAY
- Frequency of BstE II cutting?
Chris Boyd
- RNASE DECONTAMINATION REVIEW
Chris Boyd
- SWISSPROT update
Denni Schnapp
- Mac Scanner
Denni Schnapp
- Protooncogenes expression- cDNA versus RNA Probes
orlando dominguez
- help:Mac based gel Documentation systems
Chihiro Yamada
- blue colonies-no Xgal
Chris Boyd
- Mac scanner for blots...
Jim Woodgett
- Help for DNA quantification
Filip Ryniers
- MJ-Research DNA Engine
Ken Howe
- SmaI digest
MV. Hayes
- Crithidia luciliae kinetoplast. Help!
Rafel Ferragut
- Need protocol on caged cAMP
Dr. L. Darjania
- Cohesive End Ligations - Question
Kathy L
- Ab against P-ser, P-thr and P-tyr
Joe Maa
- GibcoBRL/Lifetechnologies
Ann Williams
- protein-protein interactions in vivo
Jo=?iso-8859-1?Q?=EBl BAGUET
- jnf test do not read
u.381 at inserm.u-strasbg.fr
- Library of E. coli
Namy Olivier
- RNA Extraction... ( Still )
chriso at dfrc.wisc.edu
- Human cDNA library in yeast expression vector
Guillaume Stahl
- E.coli and toxic proteins
gc
- long oligonucleotides
Matthias Vogel
- Frequency of BstE II cutting?
Mikhail Alexeyev
- Charon 4A Sequence
jjbinder at students.wisc.edu
- Subtractive cDNA
Soonmyoung Paik
- Enterovirus RT-PCR primers
Nanto Cielens
- Thermostable eukaryotes DNA libraries
Karl Voss
- QuikChange=QuickWaste?
Postmaster at p001578.icos.com
- Four-tracking from Hell; (Taq-sequencing?)
Daniel Jacobs
- MJ-Research DNA Engine
Rick Nicholson
- In situ PCR
lonnie
- Cohesive End Ligations - Question
Ned Mantei
- DNA-Elution from gel
Reinecke
- Isolation of "hot" oligos
Dr. Dietmar Kahle
- Mac scanner for blots...
a.f.hill at ic.ac.uk
- ? about DNA extraction kits
Carol Barrett
- E.coli and toxic proteins
Dr. Duncan Clark
- poly A+ purification
Adriana
- TRIZOL
thobman at anat.med.ualberta.ca
- TRIZOL
thobman at anat.med.ualberta.ca
- Rapid hybridization buffer
JOHNNY WOOD
- Elastase production
University of Palermo - Italy
- Elastase production
University of Palermo - Italy
- Elastase production
University of Palermo - Italy
- % of ammonium sulphate - PKC precipitation?
Dima Klenchin
- Mint genome size
Alex Borovkov
- Research Scientist- Molecular Biology-Signal Transduction
Krishnan Nandabalan
- Cohesive End Ligations - Question
WUSC
- SmaI digest
Michele Normore
- baculo TCR expression
Christian Radauer
- Transfection of pulmonary artery smooth muscle cells with an adenovirus vector
mahayden at med.unc.edu
- Transfection of pulmonary artery smooth muscle cells with an adenovirus vector
mahayden at med.unc.edu
- SmaI digest
Mark R. Miglarese
- Alpha-complementation in mammalian cells?
Ben Murray
- method for extracting DNA and RNA from same sample.
Terese Maltzman
- SmaI digest
TL. Borgeaud
- chloramphenicol concentration
gc
- chloramphenicol concentration
Mikhail Alexeyev
- Rifampicin activity
nico at lmb1.rug.ac.be
- failproof rt-pcr kit
Terese Maltzman
- animal and human cells - vascular and cutaneous
tedsmith at halcyon.com
- enzyme inhibition
Denni Schnapp
- Custom antibody needed
Charlie Hoffman
- RT-PCR Problems
Paul Mozdziak
- PCR with fluorescein labeled nucleotides
rlee
- protein domains
Bob Gross
- SmaI digest
Jean-Francois Larrivee
- method for extracting DNA and RNA from same sample.
Jean-Francois Larrivee
- RT-PCR Problems
John Ladasky
- Telomeric repeat amplification protocol (Kit?)
David Hieber
- TRIZOL
Chi-kuang Wen
- Tm calculation short and long oligo's
Richard Pelletier
- SmaI digest
Hernan Espinoza
- method for extracting DNA and RNA from same sample.
Rick Nicholson
- M13 Bacteriophage and Glucose
Keith Rand
- weird RNA pattern
Gene Huh
- JOB listings!!!! Web page BIOMED JOBS IN CANDA
Graham Dellaire
- Autoclaving SDS ?
Frederik Intelmann
- (none)
Karel Petrzik
- Autoclaving SDS ?
Ned Mantei
- Ficoll removal from organelle lysates
Tung Tran Duc Tuan
- Service contracts for ABI Automated DNA Sequencer
Johnjoe McFadden
- Alpha-complementation in mammalian cells?
Chris Boyd
- RT-PCR Problems
S K Green
- Subtractive cDNA
Veronique BOYER
- TRIZOL
orlando dominguez
- SmaI digest
Dr. Duncan Clark
- DEPC
Edouard Lauzier
- SmaI digest
Steven Tait
- BRL Random Priming
Steven Tait
- Inosine containing primers
Fraser Dr N J
- Help regarding availability of Mwo I restriction enzyme
Subramaniam Vadakanthara
- ELISA covers for PCR
GERALDINE KAMINSKI
- test, please ignore
Sandra Einerhand
- (no subject)
Sandra Einerhand
- anti-rat chromogranin A antibodies
Sandra Einerhand
- test, please ignore
Sandra Einerhand
- Subtractive cDNA
Sandra Pena De Ortiz
- method for extracting DNA and RNA from same sample.
Sandra Pena De Ortiz
- Gene Sequencing
Sandra Pena De Ortiz
- Methods & Reagents Newsgroups
Guivarc_h Dominic
- Mac scanner for blots...
Robin Beech
- VEGF165 cDNA needed
Heinz Rupp, PhD
- Rifampicin activity
Mikhail Alexeyev
- Source for VEGF165
Heinz Rupp, PhD
- Elisa for Q-RT-PCR
Franz Weilbach
- High background with SDS
Jens Krieger
- MJ-Research DNA Engine
Michael Casey
- % of ammonium sulphate - PKC precipitation?
Phil Robinson
- four-way ligation help !!!
Robot Boy
- MJ-Research DNA Engine
Rick Nicholson
- QIAGEN MINIPREP ROBOT
DSF
- Rep : Elisa for Q-RT-PCR
Mr SHIRE David
- Imanishi-Kari coverage
Louis Hom
- Help regarding availability of Mwo I restriction enzyme
Dr. Duncan Clark
- ELISA covers for PCR
Pete
- Rice cell cryopreservation
REGCHAK at USTHK.UST.HK
- four-way ligation help !!!
David Martin
- Help with DNA isolation
R.Peeters at nunhems.nl
- RPA's
Miss. V.M. Hodges
- GFP users in UK
Jose F Gutierrez Marcos
- four-way ligation help !!!
John Brennand
- pGEM-T cloning
Miss. V.M. Hodges
- IMPORTANT - net.bio.net down this weekend!!
BIOSCI Administrator
- QIAGEN MINIPREP ROBOT
Augustine Souza
- anti-rat chromogranin A antibodies
Dima Klenchin
- Imanishi-Kari coverage
David C. Parker
- Methylene Blue and ssDNA
Joe M Trakalo
- Adding 3' terminal thymidine to blunt ended DNA
Chi-kuang Wen
- dicistronic vector
lan zhao
- Library of E. coli
Riekeltje Koedood
- protein-protein interactions in vivo
Technical Assistance
- MJ-Research DNA Engine
George F. Mayhew
- Custom antibody needed
Guy Oshiro
- 4 way ligation avoided !!!!!!
Robot Boy
- Cohesive End Ligations - Question
Ann Williams
- Cohesive End Ligations - Question
Kathy L
- Adding 3' terminal thymidine to blunt ended DNA
Simon Q.J. RICE
- QIAGEN blood kit screwy?
Frederick Garbrecht
- method for extracting DNA and RNA from same sample.
Frederick Garbrecht
- PCR with fluorescein labeled nucleotides
Frederick Garbrecht
- RT-PCR Problems
gordon allison
- Bootscanning for recombination
Mika Salminen
- Gibco/BRL's web adress
Markus Piotrowski
- 32P-probe by PCR?
Markus Piotrowski
- Subtractive cDNA
Sandra Pena De Ortiz
- Gene Sequencing
Sandra Pena De Ortiz
- In search of a way to compare nubcleotide sequences with GenBank data.
yewks at teleview.com.sg
- Custom Antibody Needed
Signature Immunologics
- Are there labs for rent?
Allen Black
- Thermostable RNA polymerase
Takahiko Ishiguro
- 4 way ligation avoided !!!!!!
Dr. Duncan Clark
- Gibco/BRL's web adress
Rick Neubig
- ThermoSequenase
Karl Voss
- Custom oligosynthesis and custom protein sequencing
Dr.surasakdi wongratanacheewin
- Propagating errors
Michael Lonetto
- updated WWWsite: The Antibody Resource Page
Kevin Shreder
- wanted: gal4 luciferase reporter
Scott Vande Pol
- Help with DNA isolation
Michelle Gleeson
- temperature induction of BACTERIA
marcvdc at lmb1.rug.ac.be
- temperature induction in BACTERIA
marcvdc at lmb1.rug.ac.be
- rescue of tranfected DNA
Ben Mulder
- rescue of tranfected DNA
Karl Fischer
- Detection of phosphorylation in non-denaturing condition
Dooyeon Kim
- probe labeling
Carl S. Raimond III
- Help with DNA isolation
Daniel Jacobs
- Adding 3' terminal thymidine to blunt ended DNA
Sebastian Bunka
- HELP!- Protocol for THP-1: again
Daniel Fishman
- C-terminal protein sequencing
Douglas B. Kell
- In search of a way to compare nubcleotide sequences with GenBank data.
Carl Raimond
- protein-protein interactions in vivo
Jo=?iso-8859-1?Q?=EBl BAGUET
- ThermoSequenase
Dr. Duncan Clark
- Rapid hybridization buffer
Nick Jacobsen
- dicistronic vector
John Dixon
- In search of a way to compare nubcleotide sequences with GenBank data.
Klaus Salger
- Source for Aminoallyl-dUTP
LeeSylvers
- temperature induction of BACTERIA
Dr. Duncan Clark
- custom protein expression
ssieben at imb-jena.de
- ACTION ALERT Fetal Bovine Serum production.
Susan Jane Hogarth
- ELISA covers for PCR
Keld Sorensen
- Methods & Reagents Newsgroups
Keld Sorensen
- Rapid hybridization buffer
Keld Sorensen
- Tranfection of fish cells ?
b06g at unb.ca
- Fluorescent Labelled Chemokines.
Chris Moore
- IRES-Element
Matthias Vogel
- DNA quantitation
Lisa M Miller
- NEB Alkaline phosphatase contain Nuclease?
KHUANG at CHEMVX.TAMU.EDU
- QE vectors
Matthias Vogel
- Propagating errors
Gerard Tromp
- high guluronic acid alginate
Thomas J. Merchant
- 4 way ligation avoided !!!!!!
Camella Bailey
- Subtractive cDNA
Rong Wen
- Expression of toxic proteins:
- 5' nucleotide addn.
Brett Beitzel
- 4 way ligation avoided !!!!!!
Camella Bailey
- photodocumentation system?!
Biomol
- RPA's
mahayden at med.unc.edu
- PCR of bugs in berries
Walter Hill
- tips and tricks for lambda genomic library construction?
Jon Nakamoto
- triphenyltetrazolium chloride agar
Toai T. Nguyen
- TRIZOL
Lauryl Maiya J Nutter
- RT-PCR Problems
Edward Wang
- Detection of phosphorylation in non-denaturing condition
Phil Robinson
- New BIOSCI Sun server is online!
BIOSCI Administrator
- microinjecting large DNA
Robert Jordan
- Blue color comes late lately in yeast 2 hybrid
Edward K.L. Chan
- protein structure prediction
Seng-Lai Tan
- Thioredoxin fusion expression?
ijiwaru at wheel.dcn.davis.ca.us
- Yeast genome data bank access
Matthias Zeiner
- pGEM-T cloning
Christoph Grunau
- Expression of toxic proteins:
Dr. Duncan Clark
- ECL direct with oligos
Juergen Sasse (Path)
- Cytochalasin B or cytochalasin D ?
Stephane Ory
- Elisa Covers, Films for PCR plates
WSchick at aol.com
- Frequency of BstE II cutting?
Chris Boyd
- Yeast genome data bank access
Jose Miguel Lopez Coronado
- test, please ignore
Doriana Lehmann
- HELP: pcr-problems with EUROGENTEC
Doriana Lehmann
- 4 way ligation avoided !!!!!!
John Brennand
- adaptors: 5' blunt-end phosphorylated?
orlando dominguez
- Cytochalasin B or cytochalasin D ?
Herbert H. Winkler
- LDL receptor plasmids
Marc DIEU
- Thioredoxin fusion expression?
Fourie Joubert
- pGEM-T cloning
John Troyer
- Query about phage
SPIC BIOPROCESS
- c-Myc Cassettes
Troels Wind
- sequence info of pCMV,pCMVrev
lan zhao
- Rapid hybridization buffer
Martin Collinson
- Qiagen plasmid maxi kit; low yields
Miguel Rea
- MMTV PROMOTER
annalise at medmicro.uct.ac.za
- Chlorella culture media
Jorge Hernandez Torres
- T cell receptor PCR
Silvia Spitzer
- Is KlenTaq commercially available?
Scott K. Boyer
- 4 way ligation avoided !!!!!!
Klaus Salger
- PRIMERS
RBLLC at CUNYVM.CUNY.EDU
- beta gal assay
Ray Lu
- beta gal assay
Ray Lu
- P1 transductions
David Clarke
- NEB Alkaline phosphatase contain Nuclease?
Tracy Aquilla
- BRL Random Priming
Michael W. Thompson
- IMPORTANT - METHODS newsgroup index is fixed!
BIOSCI Administrator
- P1 transductions
Dr. Duncan Clark
- endotoxin kit
Marc Visconti
- IRES-Element
Emmanuel Skoufos
- Help! How to stop them?
Zhiyong Zhao
- DNA quantitation
Pamela Norton
- xylose reductase
84075
- xylose reductase
84075
- Post-ligation restriction
Richard J. Dudley
- ABI Chromosome Marker Panels
Michael C. Gorry
- Four-tracking from Hell; (Taq-sequencing?)
Daniel Mytelka
- ligation help!!
luis brieba
- DNA ligation
u.381 at inserm.u-strasbg.fr
- GFP stability in fixed tissues
Mark S. Parcells
- ligation help!!
Edgar Valencia
- Lambda Pop6
CoreyKeith
- Rapid hybridization buffer
Mark S. Whitsitt
- PRIMERS
Patrick HJ Falckh
- adaptors: 5' blunt-end phosphorilated?
orlando dominguez
- Use of RIA human insulin with ovine plasma
Value is fixed: using "Sandra.Christiansen"
- Advice on anti Ras Ab's needed
Troels Wind
- Streptavidine Magnetic Particles (SMP)
Glibert Fabrice
- PCR of bugs in berries
SG Edwards
- Help with DNA isolation
SG Edwards
- Detergents
heme at biogen.wblab.lu.se
- Anatrace
heme at biogen.wblab.lu.se
- gunk in RNA extraction
GERALDINE KAMINSKI
- RPA's
Gary Kucera
- Qiagen plasmid maxi kit; low yields
MV. Hayes
- IRES-Element
Victor Levenson
- Looking for SV40 tsA58 or tsA239 expression vector
Victor Levenson
- Lambda Ziplox
Botma Visser
- DNA quantitation
Susan Jane Hogarth
- Tm calculation short and long oligo's
kevin gilbride
- In situ hybridization
K J C 944
- PCR of bugs in berries
Dr. Duncan Clark
- Problems with one base extension cloning.
Alan KAY
- phylogenetic trees
marcvdc at lmb1.rug.ac.be
- Streptavidine Magnetic Particles (SMP)
Paul N Hengen
- Lambda Pop6
Paul N Hengen
- PCR of bugs in berries
Paul N Hengen
- Is KlenTaq commercially available?
David L. Haviland, Ph.D.
- GpppG cap and oocytes?
Laboratorio de Fisiopatologia Molecular
- Sonication
Susan Hogarth
- P1 transductions
Mikhail Alexeyev
- Help--Cultured fibroblasts come off coverslips
Brian T. Greuel
- Streptavidine Magnetic Particles (SMP)
Keld Sorensen
- Use of RIA human insulin with ovine plasma
Keld Sorensen
- Quantitative PCR
doug.perkins at mcmail.vanderbilt.edu
- Cutting glass for sequencing plates?
Roopie Toops
- Subtractive cDNA
Sandra Pena De Ortiz
- Nested Deletion Kit
Sandra Pena De Ortiz
- Tm calculation short and long oligo's
Ken Howe
- SmaI digest
John Watson
- Expression of toxic proteins:
ijiwaru at wheel.dcn.davis.ca.us
- HIV genomic RNA: polyA?
Md1214
- Expression of toxic proteins:
- anti-6Xhis antibody - any new info?
Maria C. Costanzo
- long PCR?
Kimberley Snowden
- Ethidium question
luis brieba
- DNA ligation
Richard J. Dudley
- Ethidium question
Paul N Hengen
- Quantitative PCR
Paul N Hengen
- Pointer to FAQ lists
Paul N Hengen
- Tm calculation short and long oligo's
Noel Fong
- anti-6Xhis antibody - any new info?
Hernan Espinoza
- P1 transductions
Rafael Maldonado
- NEB Alkaline phosphatase contain Nuclease?
Rafael Maldonado
- Ethidium question
Rick Thorne
- MICROEXTRACTION
dlevitzke
- Source for Anti-Betalactamase?
Charles A Miller
- about DNA recovery from agarose gel
Jin-seon Im
- E.coli and toxic proteins
I will not buy your product
- anti-6Xhis antibody - any new info?
Mark Prescott
- Biotin dUTP's
DOCON at ollamh.ucd.ie
- Help for electroporation of E.coli starved cells
Taddei Francois
- Help for electroporation of E.coli starved cells
Bregeon Damien
- Gel Shift assay
campa002 at mc.duke.edu
- Adaptors: 5' blunt-end phosphorylated?
orlando dominguez
- Help--Cultured fibroblasts come off coverslips
Dan Johnson
- Ethidium question
Nick Jacobsen
- Expression of toxic proteins:
Dr. Duncan Clark
- Tm calculation short and long oligo's
Mark Walberg
- Tm calculation short and long oligo's
campa002 at mc.duke.edu
- (none)
glycotech
- Ficoll removal from organelle lysates
Dr E. Buxbaum
- long PCR?
Bregeon Damien
- long PCR
KHUANG at CHEMVX.TAMU.EDU
- Human breakpoint region Bcl2
Isabelle Ernest
- Nigericin help
JAM
- Fos/Jun antibodies
Jennifer Anklam
- DNA Extraction From Agarose
LeeSylvers
- GpppG cap and oocytes?
Paul Digard
- 5' nucleotide addn.
Brett Beitzel
- Sure way to quantify Northern blots
Jean-Francois Larrivee
- Is anyone using 33P in RNase protection assays/Solution hybridization?
Don Martinson
- electroporation of cosmids
Dima Klenchin
- Ethidium question
Don Martinson
- electroporation of cosmids
Elke
- about DNA recovery from agarose gel
Jill Kellogg
- long PCR
Paul N Hengen
- about DNA recovery from agarose gel
Paul N Hengen
- Blue color comes late lately in yeast 2 hybrid
Riekeltje Koedood
- Blue color comes late lately in yeast 2 hybrid
Riekeltje Koedood
- Help with DNA isolation
nil
- Sure way to quantify Northern blots
Dima Klenchin
- tubes for in-situ hybridization
Anne Bazanowski
- How can I inhibit degradation of a dipeptide?
Steven Goldberg
- dephosphorylation by SAP
H. ROYCHOWDHURY
- New FDA Regs
Phillip E. Schwartz
- E.coli and toxic proteins
Steven Goldberg
- Phosphohistidine/Phosphoaspartate Antibodies
Darin B. Ostrander
- dem bones
ImmGen Inc.
- Uniequip DNA Workstation - comments?
Pam Snyder
- what is strong stop-DNA?
Robert Phelps
- BioRads InstaGene Matrix -- How does it work?
l14y at unb.ca
- Cutting glass for sequencing plates?
Michelle Gleeson
- Gibco/BRL's web adress
Roger Baird
- How can I inhibit degradation of a dipeptide?
Michael W. Thompson
- How can I inhibit degradation of a dipeptide?
Michael W. Thompson
- How can I inhibit degradation of a dipeptide?
Michael W. Thompson
- planet planaria
jgluz at STUD.MED.CORNELL.EDU
- Custom oligosynthesis and custom protein sequencing
Joan Boyce
- formalin extractions
Peter Marko
- Biotin dUTPs
Joan Boyce
- Help: Refolding of His-tagged protein on Ni-NTA.
Francisco Camara Ferreira
- Kits for H. pylori
SURASAK WONGRATANACHEEWIN
- 4 way ligation avoided !!!!!!
Gabi Gerlitz
- unbiotinylating streptavidin ... ?
Keith Hoek -BIOCHEM
- DEAE transfection
Philippe Lefebvre
- Transposons
Ed
- MICROEXTRACTION
Fraser Dr N J
- electroporation of cosmids
Fraser Dr N J
- Epilepsy: neuronal ultrastructure
Dr. T. Anoopkumar
- pBELO
Glibert Fabrice
- Test, please ignore
Antonio Rodriguez Franco
- unbiotinylating streptavidin ... ?
LeeSylvers
- DEAE transfection
John Troyer
- unbiotinylating streptavidin ... ?
Mikhail Alexeyev
- unbiotinylating streptavidin ... ?
Keld Sorensen
- PAI myeloma cell line and pseudogenes
Regina Shaw
- endotoxin kit
Technical Assistance
- PCR Mimic
Myriam Onno
- unbiotinylating streptavidin ... ?
Dima Klenchin
- Biotin dUTPs
"DAVID O'CONNELL STAFF", AGRIC. & VET. DEPT.
- shell-less CAM assay
Rene Mauricio Salazar
- tubes for in-situ hybridization
Sandra Pena De Ortiz
- semi-dry southern blotting
Coen van der Weijden
- DIG question...
Edouard Lauzier
- Non-radioactive colony hybridization
Yoonsuk Park
- Transposons
Paul N Hengen
- dephosphorylation by SAP
Dr A.H.M. Van Vliet
- Bootscanning NOTICE!
Mika Salminen
- Poly C PCR, anyone?
Brett Beitzel
- GpppG cap and oocytes?
Jillian Johnston
- Gel Shift assay
Heinz-Juergen Schaefers
- E.coli and toxic proteins
Jennifer Lyon
- Ligation with two base fill-in
Rosa Deves
- RE digest X - Ligation - RE Digest Y (purif. needed?)
Javier Alzerreca
- His- binding column
user unknown
- unsubscribe
Tom Menees
- unbiotinylating streptavidin ... ?
LeeSylvers
- reference for "Merlin" prep method
J. David Spafford
- Expression of toxic proteins:
Michael Yacuboski
- DEAE transfection
Jean-Francois Larrivee
- Poly C PCR, anyone?
Dr. Duncan Clark
- Uniequip DNA Workstation - comments?
Jeremy Carson
- Roche did not get patent on naturally occurring Taq!
Keld Sorensen
- His- binding column
Herbert H. Winkler
- Sequencing gels
Roger Dewar
- unbiotinylating streptavidin ... ?
LeeSylvers
- planet planaria
Arthur Wohlwill
- pGEM-T cloning
user unknown
- Is KlenTaq commercially available?
Robert Phillips
- Ethidium question
Administrador de iihema.fmed.uba.ar
- Is KlenTaq commercially available?
Administrador de iihema.fmed.uba.ar
- BioRads InstaGene Matrix -- How does it work?
Administrador de iihema.fmed.uba.ar
- RT-PCR PRIMERS SEQUENCE WANTED
panos zikos
- unbiotinylating streptavidin ... ?
Dima Klenchin
- pGEM-TA cloning
KHUANG at CHEMVX.TAMU.EDU
- Tm calculation short and long oligo's
Tori
- Blue color comes late lately in yeast 2 hybrid
Rafael Maldonado
- Statistical Calculation (Help! is my reasoning okay!!)
Graham Dellaire
- pGEM-T cloning
Minsoo Yoon
- How to sequence primers?
Tony Day
- Sequencing gels
Ned Mantei
- *Reliable* RPA method?
Phil Hardy
- Wanted: supF bacteria
Glenn Jenkins
- gunk in RNA extraction
Zhiyong Zhao
- Cutting glass for sequencing plates?
Rosemary T. Hoffman
- PRIMERS
Andreas Becker
- ELISA? Description please....
Rob Cowie
- Statistical Calculation (Help! is my reasoning okay!!)
Chi-Kuang Wen
- whole zebrafish cDNA library
rubind at helix.mgh.harvard.edu
- PRINS, human DNA sequences, reiteration
msms at on-luebeck.de
- RT-PCR PRIMERS SEQUENCE WANTED
Olivier Gandrillon
- pSCAN8
Antoine VEKRIS
- Taq polymerase patent (not)
Dr G R Taylor
- yeast two hybrid system
R. Woodward
- formalin extractions
Henning Lindeberg
- genomic mouse clones
FABIAN MOEBIUS
- Qiagen plasmid maxi kit; low yields
Michael Daws
- Ligation with two base fill-in
vcore
- NEED HELP: Chitinase IEF gel
Gabre Kemp
- Desperately seeking DrdI
Deborah Wilkinson
- exons & protein domains
Bob Gross
- IRES-Element
vcore
- Ericomp Thermal Cycler
Michal Shapira
- Ericomp Thermal Cycler
Michal Shapira
- Custom oligosynthesis and custom protein sequencing
Anthony Tomlinson
- Ericomp Thermal Cycler
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Michal Shapira
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immu1
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Anthony Tomlinson
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d.m. wright
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killer yeast
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killer yeast
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Arioch
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Phil Robinson
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Keld Sorensen
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killer yeast
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killer yeast
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lonnie
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HAUSER MARIE-THERES
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Dr E. Buxbaum
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Christoph H. Winter
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John Brennand
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Martin Collinson
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Dr E. Buxbaum
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Duke Groebe
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JAN CZEKAJEWSKI
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John Dixon
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Dr. Duncan Clark
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Dr. Duncan Clark
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Eirikur Sigurdsson
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Nikolai Chitaev
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Ke Wei
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Thomas Newman
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Ke Wei
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Ke Wei
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Paul N Hengen
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Mtberlin
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- Help: PCR problems with D12S99 Just wondering if anyone else has been having problems with their primer set for the D12S99 locus (12p13 region). We have been working with the probe AFM217xa7a/AFM217xa7m and have had no success, after having tried all of the following conditions: Annealing temp: 50, 55, 60 Initial Melting temp: 92, 94 Mg2+ conc: 1.5 - 4.0 mM Primer conc: 1X, 3X Glycerol/no glycerol Any more suggestions??? Thanks!!
John Wolff
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W.H. Colledge
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Stephen Morris
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William
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Pamela Norton
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Michael B. Coulthart
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Marc Lemaître
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LeeSylvers
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LeeSylvers
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RManns7
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Doriana Lehmann
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Geert Jannes
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gordon allison
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Paul N Hengen
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Paul N Hengen
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Ian J. Mehr
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JLBlackwell
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L. Bastian
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Skip
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Michael Forte
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Patrick HJ Falckh
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killer yeast
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killer yeast
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Makoto Kamei
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Rex Denton
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michael bausher
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Zhiyong Zhao
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Michael Kertesz
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chriso at dfrc.wisc.edu
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chriso at dfrc.wisc.edu
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Robot Boy
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Chanok Avirutdhakarn
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Bob Gross
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Gabriel E. DiMattia
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Namjin Chung
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Gary Kucera
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Rafael Maldonado
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John Troyer
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Majumder lab
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Ian Brandt
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Steven Brenner
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pe.com/home/netscape-galleria.htmlonnection: Keep-Alive
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Seng-Lai Tan
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killer yeast
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killer yeast
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Dr Andrew J. Doherty
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Christoph H. Winter
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Federici
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Olivier Gandrillon
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Matthias Zeiner
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WSchick at aol.com
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WSchick at aol.com
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WSchick at aol.com
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Federici
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Mikhail Alexeyev
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Richard Van Frank
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Miriam Rosin
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Richard Van Frank
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Ken Howe
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timmer at physio.emory.edu
- IMPORTANT - BIOSCI maintenance downtime 21 July
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John J. Shaskus
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David Grant
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Dr. Duncan Clark
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Tom Thatcher
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RManns7
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M C Behrendt
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Andrei Popov
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Doris Karbach
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J. Boyce
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Keld Sorensen
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Keld Sorensen
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Keld Sorensen
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martin LEACH
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Keld Sorensen
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Yihong Wang
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Rene Mauricio Salazar
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Markus Piotrowski
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Ned Mantei
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Ned Mantei
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Robin Beech
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Corrado Cilio
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David Groth
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Keith Leppard
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Chia Jin Ngee
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Enrique Graziano-Areste
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Dr Andrew J. Doherty
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Kay Junghanns
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books at mcvax2.d48.lilly.com
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Tom Dougherty
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Ole Gjoerup
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Michael W. Thompson
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John J. Shaskus
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Dr. Richard V. Goering
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S. Findley
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Harutyan Melkonyan
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Laurence Tiley
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Dr. Duncan Clark
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Joe Chou
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Christopher Dubay
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brett
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Paul N Hengen
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Narasimham Jammi
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Edouard Lauzier
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Paul N Hengen
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Paul N Hengen
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chriso at dfrc.wisc.edu
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jane staunton
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Raymond B. Jansen
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William
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Roger C. Green
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J. David Spafford
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Achim Recktenwald
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Dima Klenchin
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Roger Dewar
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David Kristofferson
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Brent Cochran
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Jan van der Ploeg
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Bernd Haase
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Chia Jin Ngee
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Chia Jin Ngee
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Timur Shtatland
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Dima Klenchin
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Russell Wrobel
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Ned Mantei
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REGCHAK at USTHK.UST.HK
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Martin Offterdinger
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Dr. Duncan Clark
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Dr. Duncan Clark
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Ulrich Tillmann
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Ulrich Tillmann
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martin LEACH
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jstrassw at opal.tufts.edu
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mazella at naxos.unice.fr
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Robert Bateman
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Richard J Treinen
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Christoph H. Winter
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campa002 at mc.duke.edu
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Ruth McMahon
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J.M. Ossewaarde
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Rich Treinen
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Namy Olivier
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Thomas J. Merchant
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Herbert H. Winkler
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Ken Howe
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Ke Wei
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Gerardo Medrano
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VanHouten, Joshua
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Corrado Cilio
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Dianne Emslie
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Zhengqiang Ma
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Chromosome Terror
- [Q] Sonication times for E. coli?
Richard Van Frank
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Daniel Jacobs
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Amie Franklin
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AShaw5
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AShaw5
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AShaw5
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Agnes Tay
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Angeline Bartholomeusz
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Zophonias O. Jonsson
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Chris Boyd
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Gary Hannan
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Chris Boyd
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orlando dominguez
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Trish Hurley
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Peter Wang
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Ke Wei
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Don Faller
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Dai Suter
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Ute Wittstock
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Ute Wittstock
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martin LEACH
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Ute Wittstock
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"Miss. V.M. Hodges", by way of
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Albert Crescenzo
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Mike Curtin
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Keld Sorensen
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Karen Posey
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Alex Chang
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Ziping Yang
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dana at zena.uokhsc.edu
- test - IGNORE
Bart Frank
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Gabriel E. DiMattia
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sgibbons at opal.tufts.edu
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Jim Woodgett
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Jill Kellogg
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little sausage called Baldrick
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Andrew C Scott
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Zheng Huanquan
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Alan Brownlee
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Angeline Bartholomeusz
- gradient pcr?
Gottfried Dasen
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Martin Offterdinger
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wick at cris.com
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RManns7
- QPCR with ABI System 7700
WSchick at aol.com
- Please HELP: Staining Glucose N-Acetyl units
Gabre Kemp
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Chris Boyd
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Ed
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Flemming Larsen
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Steven Tait
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Andy Phillips
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Jeremy Marcus
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Dieter Wiesmann
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Bart Frank
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H. ROYCHOWDHURY
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Dr A.H.M. Van Vliet
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Jory Weintraub
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Julie Roberts
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GriffCouch
- What's the best RACE system?
Dale Yuzuki
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David Bailey
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John Stebbins
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Alec Dolan
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David L. Haviland, Ph.D.
- Using ClaI for subcloning
Mikhail Alexeyev
- Computational Molecular Biology Workshop, October 15-19, 1996
Iosif Vaisman
- unsubscribe
Tanja Schwuchow
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JCULI at MVAX.CBM.UAM.ES
- ? about DNA extraction kits
benedicte Shevchenko
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Rubin Eitan
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Shane Atwell %Kuntz
- Comparing products Magazine
RManns7
- MMTV PROMOTER
Ferland Louis H.
- about DNA recovery from agarose gel
Eitan Rubin
- Taq polymerase patent (not)
Eitan Rubin
- Problems with lipids
IAN MCFARLANE
- running extracts down hi-trap columns...
Arioch
- 13C source for protein NMR
Matthew Parker
- silver staining...
Arioch
- running extracts down hi-trap columns...
Karl Fischer
- Using ClaI for subcloning
:-Peter
- silver staining...
Sailesh Surapureddi
- plaque hybridization using non-radioactive labled probes
Paul & Orit Levius
- Comparing products Magazine
Keld Sorensen
- Something living on colchicine!
Grant Morahan
- Klenow and Agarose...
Edward Wang
- Advice on anti Ras Ab's needed
Troels Wind
- Proofreading DNA polymerases and genomic DNA
WSchick at aol.com
- opinions on pCR-Script (Stratagene)
Fraser Dr N J
- spermidine/betaine/ssbp storage?
Dr. K.Hoare
- "Best" Long PCR
varyc.mmcwp4 at mmc.org
- Using ClaI for subcloning
Scott Moye-Rowley
- 13C source for protein NMR
Randall Willis
- DNA-quantification
Dieter Wiesmann
- NIH Image advice...
Paul C. Grimm MD
- Dig-riboprobes and RNase A
Kerstin Otte
- Staining gels in a microwave
Ed Beaty
- Transposon Mutagenesis HELP PLEASE
Mikhail Alexeyev
- silver staining...
Paul Digard
- PCR Machines - Suggestions?
Brian A. Flannigan
- Rat TRF?
MONSTER
- long range PCR of genomic insert in Lambda
John Dixon
- Using ClaI for subcloning
Pamela Norton
- Molecular biology software search
Dominique Larrouy
- PCR Machines - Suggestions?
Seth Corey
- Something living on colchicine!
vilimf01 at mcrcr6.med.nyu.edu
- Karen Browning?
Peta C. Bonham-Smith
- subcloning pcr bands
vilimf01 at mcrcr6.med.nyu.edu
- Transfecting A549 cells
Robin Ann Wasserman, Ph.D.
- footprint
Harutyan Melkonyan
- DNA-quantification
Harutyan Melkonyan
- Proofreading DNA polymerases and genomic DNA
vilimf01 at mcrcr6.med.nyu.edu
- site directed mutagenesis kits
M. MacDonald
- European Radiochemical suppliers
vilimf01 at mcrcr6.med.nyu.edu
- RNA blot question
Heinz-Juergen Schaefers
- gradient pcr?
martin LEACH
- RNA blot question
Heinz-Juergen Schaefers
- Molecular biology software search
Michael W. Thompson
- Need assistance re: Alkaline Elution of DNA
Kevin Henne
- Test
baba at osk.threewebnet.or.jp
- gradient pcr?
Kiley R. Prilliman
- Looking for H. Bujard's Tet system plasmids: pBI-4 ...
Eric Rheaume
- geneclean: vortexing
Trevor Bezdek
- Transfecting A549 cells
Dima Klenchin
- HOW WIDE IS ANTIBODY'S BITE?
Michael J. Tuvim
- Tetracycline inducible system
Graham Atherton
- DNA-quantification by software
Dieter Wiesmann
- Antibodies to poly-His
A. Vairis
- PCR genotyping
Dr. T.F. King
- anti HBcAg antibodies?
Graham Taylor
- European Radiochemical suppliers
John Brennand
- Protocols for determining autophosphorylation in crude plant protein extracts
AFRS
- Source for KlenTaq?
A.J.Cann
- PCR's which produce DNA concatermers
jsowden at uwa.edu.au
- PCR and seq. of microsat. region
Dr G R Taylor
- Cosmid purfication with QIAGEN minipreps
QIAGEN
- BIOSCI Archives Backlog
BIOSCI Administrator
- anti HBcAg antibodies?
Karl Fischer
- gradient pcr?
martin LEACH
- European Radiochemical suppliers
martin LEACH
- dna prep for transfection of 293 cells
Trevor Bezdek
- Concentration of DNA in Ligations???
David R. Johnson
- Source for KlenTaq
KHUANG at CHEMVX.TAMU.EDU
- Ago: AA analysis problem
Jorge Gonzalez Arroyo
- AA analysis
Jorge Gonzalez Arroyo
- An assay for alpha ketoglutarate dehydrogenase activity
Ron Caspi
- Qiagen DNA purification resin - what is is?
Dima Klenchin
- How to "normalize"
lmrc.
- How to "normalize"
lmrc.
- 13C source for protein NMR
Christoph Weber
- N-terminal His-tag expression vector
sherif at DARWIN.UCSC.EDU
- geneclean: vortexing
MELANSOND
- Test
baba at osk.threewebnet.or.jp
- carbenicillin
Torben Gjetting
- How to "normalize"
MELANSOND
- Karen Browning?
Gilles Truan
- DNA purification
Michael Kloth
- geneclean: vortexing
CAShug
- SDS PAGE and phosphorylation
Ross S Feldberg
- SDS PAGE and phosphorylation
Dima Klenchin
- QIAexpress Detection Systems
Lesley Brown
- ELISA with 4-chloro-1-naphthol
Fred Correia
- Primer Design???
Chris Troy
- ScFv expression in pVIII-coat protein??????
Danny T M Leung
- 13C source for protein NMR
Randall Willis
- N-terminal His-tag expression vector
Michael W. Thompson
- Nuclear population marker?
Bill Burnett
- Using ClaI for subcloning
Joe Boutell
- SDS PAGE and phosphorylation
Jim Woodgett
- Commenting from file
MILLER at HELIX.MGH.HARVARD.EDU
- 365nm transilluminator for photodocumentation?
Szatmari George
- Antibodies to poly-His
Toumy Guettouche
- inducible mammalian promoter
NETSCAPE APPLICATION
- Comparing products Magazine
martin LEACH
- DNA Mass Ladder problems
KARU
- RNA through the years...
H. ROYCHOWDHURY
- How to "normalize"
Heinz-Juergen Schaefers
- cross-linking protein kinase
Seng-Lai Tan
- Help with PCR library screening
Dr. Miguel A. Gomez Lim
- Gene II
David M Rivers
- SDS PAGE band blurring
Frances E. Chen
- The structure of animal genes and genomes
Phillip SanMiguel
- Detecting DNA-binding proteins
Emily Howman
- SDS PAGE band blurring
NETSCAPE APPLICATION
- Muscular dystrophy: A RANDOMIZED TRIAL OF BEMITHYL IN MUSCULAR DYSTROPHIES
STEMC
Last message date:
Wed Jul 31 23:42:19 EST 1996
Archived on: Sun Mar 6 21:06:04 EST 2005
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