100550.3012 at CompuServe.COM
Sat Jun 1 09:37:16 EST 1996
Hi all !
We experienced a few years ago that if DNA was mixed with low grade phenol
and even if you removed the phenol with several ethers or chloroforms....
The DNA was impossible to cut ! We solved this problem with using some
more expensive phenol.
We have now another problem. It seems that when you mix your DNA (PCR
products of about 3 kb) with a new phenol (that is supposed to be
equilibrated in T.E.) you loose the DNA !!! It worked just fine for years
and now it looks like it could come from a new phenol batch. Did anybody
experienced such problems ? Or what is your idea about that ? All
suggestions are welcome ! (The first idea is that we will not use that
phenol any longer !!!).
Thank for your help.
Philippe ROUET, PHD
INSERM U 78
76233 BOISGUILLAUME Cedex
E-mail: ROUETP at AOL.COM
E-mail: 100550.3012 at Compuserve.com
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