Hi,there,
Could anyone help me solve the DNA ligation problem? THe Case below:
Insert: 16.3kb SalI cut
Vector: Bscript KS or other expression vector SalI cut
Want to transfer to E.Coli DH5a cell
I could not success ligate and transfer them no matter using chemical
or electroparation method,
Did anyone have ever met the same case? any suggestion will be
appreciated!