DNA ligation help

chunlin xin xcl at med.unc.edu
Sat Jun 1 15:07:05 EST 1996

 Could anyone help me solve the DNA ligation problem? THe Case below:
       Insert: 16.3kb SalI cut 
       Vector: Bscript KS or other expression vector SalI cut
       Want to transfer to E.Coli DH5a cell
 I could not success ligate and transfer them no matter using chemical
or electroparation method,
  Did anyone have ever met the same case? any suggestion will be 

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