pcr problem with gc rich sequences

gwen jenkins aaa186 at agora.ulaval.ca
Sun Jun 2 19:18:27 EST 1996


We are trying to amplify a gene with many repeats in GC rich
sequences. After PCR, no amplification seems to appear. I've heard
that the Polymerase is blocked in some way by secondary structures. We
have tried SSB proteins  to stabilize it but no improvement was
observed. Is somobody know a way to do it ? If so please contact us by
e-mail at BHXM at musicb.mcgill.ca

Stephane Angers





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