Cloning Vectors

Jason Curole JCUROLE at LSUVM.SNCC.LSU.EDU
Sun Jun 2 19:53:41 EST 1996


To all:
We are interested in setting up TA Cloning in our laboratory.  I have seen
the kit from Invitrogen (and others), but would prefer to buy the components
separately and make my own T-vector (sans. Marchuk et. al. Nucleic Acids
Research 19:1154).

My first question is: Does anyone out there have any experience with making
T-vectors this way?  Marchuk et. al. make the procedure sound rather simple
and claim to have great sucess with it (9 of 9 PCR products were successfully
cloned).  Is it really this easy?

Secondly, Marchuck et. al. used Stratagene's Bluescript vector, but claim
any vector with a unique blunt end restriction site will work.  Is there any
reason to use Stratagene's vectors over, say, Promega or Gibco's vectors?

If there is an FAQ or any previous discussion of this topic on the net
please let me know.  Feel free to e-mail me directly:
jcurole at lsuvm.sncc.lsu.edu

Thank you

JASON P. CUROLE
DEPARTMENT OF ZOOLOGY AND PHYSIOLOGY
LOUISIANA STATE UNIVERSITY
BATON ROUGE LA 70803-1725



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