SEAP assay

Protein Chemistry Facility ajpchem at
Sun Jun 2 17:00:55 EST 1996

In article <thompsop-2905961612510001 at>,
thompsop at (Phillip R Thompson) wrote:

> I am using Clontech's Great Escape SEAP Genetic reporter system & am
> having a major problem with reproducibility.  Some of my duplicates differ
> by as much as 300%!  The assays are being carried out essentially as
> described in the manual & read in 96-well black plates in a Wallach plate
> reader.  I'm being very careful with pipetting & I don't believe pipetting
> error could result in such large errors anyway.  Any help would be
> appreciated!
I don't know the details of the Clontech protocol, I have used one from
Tropix Inc. . Killing the endogenous alkaline phosphatase by heating at 65
C is essential though. I do this directly in the 96 well plate in a hyb.
Hope this helps, 
Breandan Kennedy,
Protein Chemistry Facility,
L. Placid, 
NY 12946
Ph  518-523-1268
fax 518-523-4970/1849

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