DEAE-dextran transfection/divalent cations

Graham Dellaire dellaire at ODYSSEE.NET
Mon Jun 3 21:03:35 EST 1996


Hello Nico,


I transfect cells (murine fibroblasts) with DEAE-dextran and I don't 
worry about Ca or Mg ion content.  It works fine... I found the mol 
weight of the DEAE-dextran you use has more of an effect.  Try to get 
100,000 or higher.


G. Dellaire
dellaire at odyssee.net



More information about the Methods mailing list