DEAE-dextran transfection/divalent cations

Graham Dellaire dellaire at ODYSSEE.NET
Mon Jun 3 21:03:35 EST 1996

Hello Nico,

I transfect cells (murine fibroblasts) with DEAE-dextran and I don't 
worry about Ca or Mg ion content.  It works fine... I found the mol 
weight of the DEAE-dextran you use has more of an effect.  Try to get 
100,000 or higher.

G. Dellaire
dellaire at

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