Acrylamide Gel setting too quickly -- IDEAS??

Achim Recktenwald achim at ibex.ca
Mon Jun 3 10:00:51 EST 1996


Brian MacNevin wrote:
> 
> Well, when I mix up my acrylamide gel solution and begin pipetting it
> between the glass plates for electrophoresis, I have found that it
> starts to set before I can get it evenly dispersed in the plates.
>         My first thought is that this might be due to the temp of the
> solution.  It seems to me that I don't have this problem if the
> solution is allowed to cool a little before adding the final solution.
> I was wonderin if anyone else has had these problems and if it really
> is due to the temp of the solution?
>         Thanks!
>                 Brian
>                 macnevin at ix.netcom.com

How much TEMED are you using ?   Try with half the amount.

Achim



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