Microinjection of Murine ES cells... any experiences? What is involved?

[Graham Dellaire]

Hello All,

I am thinking of trying microinjection of mouse ES cells and I was 
wondering what is involved as far as equipement (including rough 
pricing??) and time.  I have seen papers where they inject several 
thousand cells (many a paper by Capecchi) and I was wondering how 
difficult this is to do.  We routinely inject pronuclei in mouse 
oocytes.  Is it much more involved than this?

Any and all hints, suggestions are appreciated



G. Dellaire
McGill dept of Medicine
Div. of Experimental Medicine.

dellaire at odyssee.net