repetitive dna

joerg hauf hauf at bio1.bio.th-darmstadt.de
Mon Jun 3 06:17:45 EST 1996


Hello everybody,

is there anyone experienced in cloning repetitive DNA? I've tried to 
clone tandemly repeated DNA in E. coli strains Sure and DH5alpha several 
times, but neither of them contained the desired plasmid.
The Sure strain produced no clones at all, DH5alpha produced only 
plasmids with wrong insertions.
My question is: Does anybody know of any other strain which may be used 
for that purpose?




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