Cell cycle analysis by FACS
kmorris1 at opal.tufts.edu
Tue Jun 4 15:49:56 EST 1996
I've done cell cycle analysis on a FACS before, but always with fresh cells.
Now I'm setting up a time course (block HeLa cells with nocodazole 24 hours,
release, 4 hour timepoints) and along with RNA and protein isolation I want to
do cell cycle analysis. How do I prepare/store the cells at each time point so
that I can do the analysis at a later time? This is sort of a last-minute
addition to this experiment and I won't be able to get FACS time for at least a
week or two (I hate crowded machines :-). Do I just freeze the cell pellet?
If someone has experience with this sort of thing, I'd appreciate a protocol
or some suggestions. Thank you very much!
Kelly Thome Ph.D. kmorris1 at opal.tufts.edu
Pathology Department fax: 617-732-7449
Brigham and Women's Hospital
Boston, MA 02115 <insert standard disclaimer here>
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