Help:Stratagene and MORPH mutagenesis kits
A.S. Sohal
u9310871 at MUSS.CIS.MCMASTER.CA
Tue Jun 4 12:43:00 EST 1996
Hi! My name is Avtar. I am experimenting with the MORPH single-primer
site-directed mutagenesis kit and the Chameleon double-stranded
site-directed mutagenesis kit from Stratagene. I was wondering if
anybody has been able to get these protocols to work. I am having
trouble isolating my plasmid after transformation into the mut S cells
using a standard alkaline lysis mini-prep protocol. I hopt someone can
help me.
Thanx
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