Minipreps for ABI sequencing
michelle at MOLECULE.BIO.UTS.EDU.AU
Wed Jun 5 00:15:48 EST 1996
On Tue, 4 Jun 1996, J. David Spafford wrote:
> I have been using Qiagen minipreps for sequencing with good results. PEG
> precipitation also works well.
> I have been offered to try a cheaper kit from PROMEGA, a Wizard miniprep kit
> specially designed for ABI sequencing quality DNA. Has anyone have any luck
> with it? How does it compare to Qiagen?
> Thanks in advance,
> J. David Spafford,
> Department of Biological Sciences, University of Alberta
> jspaffor at gpu.srv.ualberta.ca
A sequencing facility I used recommended an extra ethanol precipitation
step at the end of the column purification to remove salts. This applied
to all column systems, including Wizards and Qiagens. I used the standard
Wizard miniprep kit as I had it on hand, did the extra precipitation step,
and the sequence was great. The "new" kit may be nothing more than a
slight modification of the standard kit protocol, compare them and see.
michelle.gleeson at uts.edu.au
More information about the Methods