Help: plasmid for functional expression

Mark A Osinski osins001 at maroon.tc.umn.edu
Tue Jun 4 19:53:14 EST 1996


I finally get all of my receptor with RACE PCR, ligate it into Promega's 
T vector (pGEM-5Zf+) and prepare plasmid preps.  I'm about to make cRNA 
for Xenopus oocyte injection, when I realize that the polylinker region 
between the RNA polymerase promoters contains two start codons (ATG) 
upstream of the insert and two more start codons downstream.  Is this 
going to be a problem?  Any help will be greatly appreciated.

Thanks in advance

Mark Osinski

osins001 at maroon.tc.umn.edu



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