Phenol Stories

[Patrick HJ Falckh]

-Some of the 'new 'phenols are buffered differently.  As far as I 
remember, but not the reference, slightly acidic phenol is best for 
RNA and slightly basic best for DNA - some companies now make the 
phenols at ~pH6.6 and ~pH7.8.  This slight variation in pH will make 
quite a bit of difference to your extraction.

Best Regards
- 
                                                                    
   Patrick HJ Falckh PhD                                 #     #     
   Key Centre for Applied & Nutritional Toxicology      # # # # #    
   RMIT - City Campus                                    # Q Q #     
   GPO Box 2467V, Melbourne  Vic  3001                   #  o  #     
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   Telephone  : 03 9660 3136                             ##<->##     
   Fax No.    : 03 9663 6087                            #       #    
   Email      : p.falckh at rmit.edu.au                   # #     # #   
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   "No matter how tall your grandfather                  #  \/  #    
       was, you have to do your own growing"              # /\ #
                                                         ooO  Ooo