SDS & Chelex
comstock at plains.nodak.edu
Tue Jun 4 17:32:58 EST 1996
I have been working with spiders trying to isolate genomic DNA for RAPD
analysis using two approaches:
1). standard phenol chloroform with SDS and Proteinase K
2). Chelex-100 Resin by Biorad with a Proteinase K digestion, then a
My question is does anyone know what effects I'll have if I use SDS in my
Chelex extraction if I don't get rid of it before PCR? Any information
would be helpful.
e-mail comstock at plains.nodak.edu
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