SDS & Chelex
Clay Comstock
comstock at plains.nodak.edu
Tue Jun 4 17:32:58 EST 1996
Hello,
I have been working with spiders trying to isolate genomic DNA for RAPD
analysis using two approaches:
1). standard phenol chloroform with SDS and Proteinase K
digestion.
2). Chelex-100 Resin by Biorad with a Proteinase K digestion, then a
30min. boil.
My question is does anyone know what effects I'll have if I use SDS in my
Chelex extraction if I don't get rid of it before PCR? Any information
would be helpful.
Thanks,
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Clay Comstock
e-mail comstock at plains.nodak.edu
homepage http://murphy220.phys.dsu.nodak.edu/students/clay/comstock.htm
PGP Public Key available through most key servers.
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