SDS & Chelex

Clay Comstock comstock at plains.nodak.edu
Tue Jun 4 17:32:58 EST 1996


Hello,

I have been working with spiders trying to isolate genomic DNA for RAPD 
analysis using two approaches:

1). standard phenol chloroform with SDS and Proteinase K 
digestion. 

2). Chelex-100 Resin by Biorad with a Proteinase K digestion, then a 
30min. boil.

My question is does anyone know what effects I'll have if I use SDS in my 
Chelex extraction if I don't get rid of it before PCR?  Any information 
would be helpful.

Thanks,
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Clay Comstock
e-mail comstock at plains.nodak.edu     
homepage http://murphy220.phys.dsu.nodak.edu/students/clay/comstock.htm
PGP Public Key available through most key servers.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

                      
                                         
               
                  
                                                 
                 



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