Acrylamide Gel setting too quickly -- IDEAS??

[Harry Reis]

michelle quoted on 02.06.96:


>
>
> On Sat, 1 Jun 1996, Brian MacNevin wrote:
>
> > 	My first thought is that this might be due to the temp of the
> > solution.  It seems to me that I don't have this problem if the
> > solution is allowed to cool a little before adding the final solution.
> >
> >
> >
> Dear Brian,
> Yes, it is definitely the temperature.  Acrylamide gels always take
> longer to set on cold days.  Try chilling the acrylamide mix before adding
> the TEMED and  APS until it feels cool to touch.  Also, don't use high heat
> to dissolve the urea.  It will take a little longer but not nearly as long
> as preparing a new set of plates!  Good luck,
> Michelle
> michelle.gleeson at uts.edu.au

It's also helpful to put the glassplates in the fridge for about fifteen  
minutes.


... see you later aligator



                                   Harry