Which bacterial species support pUC18/19 replication?

Michael Benedik benedik at uh.edu
Tue Jun 4 10:21:03 EST 1996


In article <4p1jgh$lg8_001 at lwli281.rivm.nl>
Karel.Wernars at rivm.nl (Karel Wernars) writes:

> Dear netters,
> 
> A few days ago I was asked by a colleague if I had any information on 
> the hostrange of pUC18/19 plasmids. Can these replicate in bacteria 
> other then E. coli?. To my idea various enterobacteriaceae can support 
> replication e.g. Salmonella and Vibrio.
> 
> Digging through literature didn't help me much to confirm this, and I 
> only came across lots of reports on modifications of pUC18/19 to prepare 
> shuttle vectors that would replicate in a pacticular bacterial host of 
> interest.
> 
> Can anyone help me with this question and are there any studies 
> available on the host range of (unmodified) pUC18/19?.
> 
> Thanks,
> Karel Wernars (Karel.Wernars at rivm.nl)

They will replicate in many enterobacteriacea. They will replicate in
Shigella, Salmonella, Serratia. I believe they won't in Vibrio (not
positive though). I don't know about the others. 

Michael Benedik
Department of Biochemical Sciences
University of Houston
benedik at uh.edu



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