Acrylamide Gel setting too quickly -- IDEAS??

[Michael Benedik]

In article <31B2FE23.6FEF at ibex.ca>
Achim Recktenwald <achim at ibex.ca> writes:

> Brian MacNevin wrote:
> > 
> > Well, when I mix up my acrylamide gel solution and begin pipetting it
> > between the glass plates for electrophoresis, I have found that it
> > starts to set before I can get it evenly dispersed in the plates.
> >         My first thought is that this might be due to the temp of the
> > solution.  It seems to me that I don't have this problem if the
> > solution is allowed to cool a little before adding the final solution.
> > I was wonderin if anyone else has had these problems and if it really
> > is due to the temp of the solution?
> >         Thanks!
> >                 Brian
> >                 macnevin at ix.netcom.com
> 
> How much TEMED are you using ?   Try with half the amount.
> 
> Achim

You are both correct. Temperature will affect the rate, let your
solution cool to room temp. Also the amount of TEMED will affect the
rate.


Michael Benedik
Department of Biochemical Sciences
University of Houston
benedik at uh.edu