Single-stranded DNA stability

Karen Nel karen at
Tue Jun 4 16:26:05 EST 1996

Could someone please advise?

We are using RT-PCR to amplify genes, so we reverse-transcribe mRNA to 
single-stranded DNA and then use this for PCR.  The success of the PCR 
reactions is reduced over a period of time and we think this may have to do 
with the stability of the single-stranded DNA.  We have tried heating the 
sample in case the DNA has stuck to the tubes but this seems to make no 
difference.  Does anyone have any information or advice on storage of ss DNA?

Thank you in advance

Karen Nel                                             karen at
Department of Biochemistry
University of Pretoria

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